Publications des scientifiques de l'IRD

Elleuch J., Jacca S., Darriet Frédéric, Chandre Fabrice, Tounsi S., Zghal R. Z. (2015). Cry4Ba and Cyt1Aa proteins from Bacillus thuringiensis israelensis : interactions and toxicity mechanism against Aedes aegypti. Toxicon, 104, p. 83-90. ISSN 0041-0101.

Titre du document
Cry4Ba and Cyt1Aa proteins from Bacillus thuringiensis israelensis : interactions and toxicity mechanism against Aedes aegypti
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000361777800013
Auteurs
Elleuch J., Jacca S., Darriet Frédéric, Chandre Fabrice, Tounsi S., Zghal R. Z.
Source
Toxicon, 2015, 104, p. 83-90 ISSN 0041-0101
Individual crystal proteins from Bacillus thuringiensis israelensis exhibit variable levels of insecticidal activities against mosquito larvae. In all cases, they are much less active compared to the whole crystal proteins due to described complex synergistic interactions among them. In the present study we investigated the effects of Cyt1A98 (a Cyt1Aa type protein) on Cry4BLB (a Cry4Ba type toxin) insecticidal activity toward the dengue vector Aedes aegypti. The bioassay analyses demonstrated the ability of Cyt1A98 protein to enhance Cry4BLB toxin larvicidal activity even at a low proportion in the mixture (1%). In vitro interaction assays showed that Cyt1A98 provides supplementary binding sites for Cry4BLB in A. aegypti BBMVs. Moreover, it enhances the formation of Cry4BLB oligomeric structure. These results support that Cyt1A98 protein could act as a membrane-bound receptor fixing Cry4BLB delta-endotoxins and promoting its oligomerization.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
Localisation
Fonds IRD [F B010065326]
Identifiant IRD
fdi:010065326
Contact