@article{fdi:010065277,
  title = {{A} metagenomic investigation of the duodenal microbiota reveals links with obesity},
  author = {{A}ngelakis, {E}. and {A}rmougom, {F}abrice and {C}arriere, {F}. and {B}achar, {D}. and {L}augier, {R}. and {L}agier, {J}. {C}. and {R}obert, {C}. and {M}ichelle, {C}. and {H}enrissat, {B}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {F}ew studies have tested the small intestine microbiota in humans, where most nutrient digestion and absorption occur. {H}ere, our objective was to examine the duodenal microbiota between obese and normal volunteers using metagenomic techniques. {M}ethodology/{P}rincipal {F}indings {W}e tested duodenal samples from five obese and five normal volunteers using 16{S} r{DNA} {V}6 pyrosequencing and {I}llumina {M}i{S}eq deep sequencing. {T}he predominant phyla of the duodenal microbiota were {F}irmicutes and {A}ctinobacteria, whereas {B}acteroidetes were absent. {O}bese individuals had a significant increase in anaerobic genera (p < 0.001) and a higher abundance of genes encoding {A}cyl-{C}o{A} dehydrogenase (p = 0.0018) compared to the control group. {O}bese individuals also had a reduced abundance of genes encoding sucrose phosphorylase (p = 0.015) and 1,4-alpha-glucan branching enzyme (p = 0.05). {N}ormal weight people had significantly increased {F}ab{K} (p = 0.027), and the glycerophospholipid metabolism pathway revealed the presence of phospholipase {A}1 only in the control group (p = 0.05). {C}onclusions/{S}ignificance {T}he duodenal microbiota of obese individuals exhibit alterations in the fatty acid and sucrose breakdown pathways, probably induced by diet imbalance.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {10},
  numero = {9},
  pages = {e0137784 [15 p.]},
  ISSN = {1932-6203},
  year = {2015},
  DOI = {10.1371/journal.pone.0137784},
  URL = {https://www.documentation.ird.fr/hor/fdi:010065277},
}