@article{fdi:010065251,
  title = {{I}dentifying genomic changes associated with insecticide resistance in the dengue mosquito {A}edes aegypti by deep targeted sequencing},
  author = {{F}aucon, {F}. and {D}usfour, {I}. and {G}aude, {T}. and {N}avratil, {V}. and {B}oyer, {F}. and {C}handre, {F}abrice and {S}irisopa, {P}. and {T}hanispong, {K}. and {J}untarajumnong, {W}. and {P}oupardin, {R}. and {C}hareonviriyaphap, {T}. and {G}irod, {R}. and {C}orbel, {V}incent and {R}eynaud, {S}. and {D}avid, {J}. {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he capacity of mosquitoes to resist insecticides threatens the control of diseases such as dengue and malaria. {U}ntil alternative control tools are implemented, characterizing resistance mechanisms is crucial for managing resistance in natural populations. {I}nsecticide biodegradation by detoxification enzymes is a common resistance mechanism; however, the genomic changes underlying this mechanism have rarely been identified, precluding individual resistance genotyping. {I}n particular, the role of copy number variations ({CNV}s) and polymorphisms of detoxification enzymes have never been investigated at the genome level, although they can represent robust markers of metabolic resistance. {I}n this context, we combined target enrichment with high-throughput sequencing for conducting the first comprehensive screening of gene amplifications and polymorphisms associated with insecticide resistance in mosquitoes. {M}ore than 760 candidate genes were captured and deep sequenced in several populations of the dengue mosquito {A}e. aegypti displaying distinct genetic backgrounds and contrasted resistance levels to the insecticide deltamethrin. {CNV} analysis identified 41 gene amplifications associated with resistance, most affecting cytochrome {P}450s overtranscribed in resistant populations. {P}olymorphism analysis detected more than 30,000 variants and strong selection footprints in specific genomic regions. {C}ombining {B}ayesian and allele frequency filtering approaches identified 55 nonsynonymous variants strongly associated with resistance. {B}oth {CNV}s and polymorphisms were conserved within regions but differed across continents, confirming that genomic changes underlying metabolic resistance to insecticides are not universal. {B}y identifying novel {DNA} markers of insecticide resistance, this study opens the way for tracking down metabolic changes developed by mosquitoes to resist insecticides within and among populations.},
  keywords = {{THAILANDE} ; {AMERIQUE} {DU} {SUD}},
  booktitle = {},
  journal = {{G}enome {R}esearch},
  volume = {25},
  numero = {9},
  pages = {1347--1359},
  ISSN = {1088-9051},
  year = {2015},
  DOI = {10.1101/gr.189225.115},
  URL = {https://www.documentation.ird.fr/hor/fdi:010065251},
}