Publications des scientifiques de l'IRD

Séré M. G., Tortosa P., Chabanet Pascale, Quod J. P., Sweet M. J., Schleyer M. H. (2015). Identification of a bacterial pathogen associated with Porites white patch syndrome in the Western Indian Ocean. Molecular Ecology, 24 (17), p. 4570-4581. ISSN 0962-1083.

Titre du document
Identification of a bacterial pathogen associated with Porites white patch syndrome in the Western Indian Ocean
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000360445700017
Auteurs
Séré M. G., Tortosa P., Chabanet Pascale, Quod J. P., Sweet M. J., Schleyer M. H.
Source
Molecular Ecology, 2015, 24 (17), p. 4570-4581 ISSN 0962-1083
Porites white patch syndrome (PWPS) is a coral disease recently described in the Western Indian Ocean. This study aimed to isolate and identify potential pathogens associated with PWPS utilizing both culture and nonculture screening techniques and inoculation trials. A total of 14 bacterial strains (those dominant in disease lesions, absent or rare in healthy tissues and considered potential pathogens in a previous study) were cultured and used to experimentally inoculate otherwise healthy individuals in an attempt to fulfil Henle-Koch's postulates. However, only one (P180R), identified as closely related (99-100% sequence identity based on 1.4kb 16S RNA sequence) to Vibrio tubiashii, elicited signs of disease in tank experiments. Following experimental infection (which resulted in a 90% infection rate), the pathogen was also successfully re-isolated from the diseased tissues and re-inoculated in healthy corals colonies, therefore fulfilling the final stages of Henle-Koch's postulates. Finally, we report that PWPS appears to be a temperature-dependent disease, with significantly higher tissue loss (anova: d.f.=2, F=39.77, P<0.01) occurring at 30 degrees C [1.45 +/- 0.85cm(2) per day (mean +/- SE)] compared to ambient temperatures of 28 and 26 degrees C (0.73 +/- 0.80cm(2) per day (mean +/- SE) and 0.51 +/- 0.50cm(2) per day (mean +/- SE), respectively).
Plan de classement
Ecologie, systèmes aquatiques [036]
Description Géographique
OCEAN INDIEN ; REUNION ; MAYOTTE
Localisation
Fonds IRD [F B010064947]
Identifiant IRD
fdi:010064947
Contact