@article{fdi:010064945,
  title = {{M}icrobial diversity in a submarine carbonate edifice from the serpentinizing hydrothermal system of the {P}rony {B}ay ({N}ew {C}aledonia) over a 6-year period},
  author = {{P}ostec, {A}. and {Q}u{\'e}m{\'e}neur, {M}arianne and {B}es, {M}. and {M}ei, {N}. and {B}enaissa, {F}. and {P}ayri, {C}laude and {P}elletier, {B}ernard and {M}onnin, {C}. and {G}uentas-{D}ombrowsky, {L}inda and {O}llivier, {B}ernard and {G}erard, {E}mmanuelle and {P}isapia, {C}. and {G}{\'e}rard, {M}artine and {M}enez, {B}. and {E}rauso, {G}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}ctive carbonate chimneys from the shallow marine serpentinizing {P}rony {H}ydrothermal {F}ield were sampled 3 times over a 6 years period at site {ST}09. {A}rchaeal and bacterial communities composition was investigated using {PCR}-based methods (clone libraries, {D}enaturating {G}el {G}radient {E}lectrophoresis, quantitative {PCR}) targeting 16{S} r{RNA} genes, methyl coenzyme {M} reductase {A} and dissimilatory sulfite reductase subunit {B} genes. {M}ethanosarcinales ({E}uryarchaeota) and {T}haumarchaea were the main archaeal members. {T}he {M}ethanosarcinales, also observed by epifluorescent microscopy and {FISH}, consisted of two phylotypes that were previously solely detected in two other serpentinitzing ecosystems ({T}he {C}edars and {L}ost {C}ity {H}ydrothermal {F}ield). {S}urprisingly, members of the hyperthermophilic order {T}hermococcales were also found which may indicate the presence of a hot subsurface biosphere. {T}he bacterial community mainly consisted of {F}irmicutes, {C}hloroflexi, {A}lpha-, {G}amma-, {B}eta-, and {D}elta-proteobacteria and of the candidate division {NPL}-{UPA}2. {M}embers of these taxa were consistently found each year and may therefore represent a stable core of the indigenous bacterial community of the {PHF} chimneys. {F}irmicutes isolates representing new bacterial taxa were obtained by cultivation under anaerobic conditions. {O}ur study revealed diverse microbial communities in {PHF} {STO}9 related to methane and sulfur compounds that share common populations with other terrestrial or submarine serpentinizing ecosystems.},
  keywords = {hydrothermal ; serpentinization ; alkaline ; {P}rony ; microbial communities ; {NOUVELLE} {CALEDONIE} ; {PACIFIQUE} ; {PRONY} {BAIE}},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {6},
  numero = {},
  pages = {art. 857 [19 p.]},
  ISSN = {1664-302{X}},
  year = {2015},
  DOI = {10.3389/fmicb.2015.00857},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064945},
}