@article{fdi:010064917,
  title = {{A}ssessment of genetic and epigenetic changes during cell culture ageing and relations with somaclonal variation in {C}offea arabica},
  author = {{L}andey, {R}. {B}. and {C}enci, {A}lberto and {G}uyot, {R}omain and {B}ertrand, {B}. and {G}eorget, {F}. and {D}echamp, {E}. and {H}errera, {J}. {C}. and {A}ribi, {J}amel and {L}ashermes, {P}hilippe and {E}tienne, {H}.},
  editor = {},
  language = {{ENG}},
  abstract = {{L}ong-term cell cultures were used in coffee to study the cytological, genetic and epigenetic changes occurring during cell culture ageing. {T}he objective was to identify the mechanisms associated with somaclonal variation ({SV}). {T}hree embryogenic cell lines were established in {C}offea arabica (2n = 4x = 44) and somatic seedlings were regenerated after 4, 11 and 27 months. {P}henotyping and {AFLP}, {MSAP}, {SSAP} molecular markers were performed on 199 and 124 plants, respectively. {SV} were only observed from the 11 and 27-month-old cultures, affecting 30 and 94 % of regenerated plants, respectively. {C}hromosome counts performed on 15 plants showed that normal plants systematically displayed normal chromosome numbers and that, conversely, aneuploidy (monosomy) was systematically found in variants. {T}he allopolyploid structure of {C}. arabica allowed aneuploid cells to survive and regenerate viable plants. {N}o polymorphic fragments were observed between the {AFLP} and {SSAP} electrophoretic profiles of mother plants and those of the in vitro progeny. {M}ethylation polymorphism was low and ranged between 0.087 and 0.149 % irrespective of the culture age. {T}he number of methylation changes per plant-normal or variant-was limited and ranged from 0 (55-80 % of the plants) to 4. {T}he three cell lines showed similar {SV} rate increases during cell culture ageing and produced plants with similar molecular patterns indicating a non random process. {T}he results showed that cell culture ageing is highly mutagenic in coffee and chromosomal rearrangements are directly linked to {SV}. {C}onversely, the analysis of methylation and transposable elements changes did not reveal any relation between the epigenetic patterns and {SV}.},
  keywords = {{C}hromosome number ; {DNA} methylation ; {G}enetic and epigenetic stability ; {L}ong-term cell culture ; {M}olecular markers ; {T}ransposable elements},
  booktitle = {},
  journal = {{P}lant {C}ell {T}issue and {O}rgan {C}ulture},
  volume = {122},
  numero = {3},
  pages = {517--531},
  ISSN = {0167-6857},
  year = {2015},
  DOI = {10.1007/s11240-015-0772-9},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064917},
}