@article{fdi:010064873,
  title = {{S}acha {I}nchi {O}il ({P}lukenetia volubilis {L}.), effect on adherence of {S}taphylococus aureus to human skin explant and keratinocytes in vitro},
  author = {{G}onzalez-{A}spajo, {G}. and {B}elkhelfa, {H}. and {H}addioui-{H}babi, {L}. and {B}ourdy, {G}enevi{\`e}ve and {D}eharo, {E}ric},
  editor = {},
  language = {{ENG}},
  abstract = {{E}thnopharmacological relevance: {P}lukenetia volubilis {L}. ({E}uphorbiaceae) is a domesticated vine distributed from the high-altitude {A}ndean rain forest to the lowlands of the {P}eruvian {A}mazon. {O}il from the cold-pressed seeds, sold under the commercial name of {S}acha {I}nchi {O}il ({SIO}) is actually much in favour because it contains a high percentage of omega 3 and omega 6, and is hence used as a dietary supplement. {SIO} is also used traditionally for skin care, in order to maintain skin softness, and for the treatment of wounds, insect bites and skin infections, in a tropical context where the skin is frequently damaged. {A}ims of the study: {T}his study was designed in order to verify whether the traditional use of {SIO} for skin care would have any impact on {S}taphylococcus aureus growth and skin adherence, as {S}. aureus is involved in many skin pathologies (impetigo, folliculitis, furuncles and subcutaneous abscesses) being one if the main pathogens that can be found on the skin. {T}herefore, our objective was to assess {SIO} bactericidal activity and interference with adherence to human skin explants and the keratinocyte cell line. {C}ytotoxicity on that cells was also determined. {T}he activity of {SIO} was compared to coconut oil ({C}oc{O}), which is widely used for skin care but has different unsaturated fatty acids contents. {M}aterials and methods: {L}aboratory testing with certified oil, determined antibacterial activity against radio labelled {S}. aureus. {C}ytotoxic effects were measured with {XTF} on keratinocyte cells and with neutral red on human skin explants; phenol was used as cytotoxic control. {A}dherence assays were carried out by mixing {H}3-labelled {S}. aureus bacteria with keratinocyte cells and human skin explants, incubated with oils 2 h before (to determine the inhibition of adherence, assimilated to a preventive effect) or 2 h after the contact of the biological material with {S}. aureus (to assess the detachment of the bacteria, assimilated to a curative effect). {R}esidual radioactivity measured after washings made it possible to determine the adherence intensity. {B}actericidal effect was determined by colony counting on trypticase soy agar. {R}esults: {L}aboratory assays showed that {SIO} and {C}oc{O}, tested undiluted, were not cytotoxic on keratinocytes nor human explants and were not bactericidal neither. {SIO} was more active as antiadherent (preventive) than {C}oc{O} on keratinocytes. {T}here was no significant difference between detachment effects (curative) of both oils on keratinocytes but {SIO} was almost 5 times more active on the detachment of {S}. aureus from human skin explants. {C}onclusion: {F}rom that study it can be concluded that the use of {SIO} on dermal cells is safe and efficient in the inhibition of {S}. aureus adherence. {O}ur results tend to support the traditional use of undiluted {SIO} in skin care.},
  keywords = {{S}acha {I}nchi {O}il ; {S}taphylococcus aureus ; {H}uman skin explant ; {K}eratinocytes cells ; {P}lukenetia volubilis {L}. ; {PEROU} ; {AMAZONIE} ; {ANDES}},
  booktitle = {},
  journal = {{J}ournal of {E}thnopharmacology},
  volume = {171},
  numero = {},
  pages = {330--334},
  ISSN = {0378-8741},
  year = {2015},
  DOI = {10.1016/j.jep.2015.06.009},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064873},
}