@article{fdi:010064852, title = {{V}iruses occur incorporated in biogenic high-{M}g calcite from hypersaline microbial mats}, author = {{D}e {W}it, {R}. and {G}autret, {P}. and {B}ettarel, {Y}van and {R}oques, {C}. and {M}arli{\`e}re, {C}. and {R}amonda, {M}. and {T}hanh, {T}. {N}. and {Q}uang, {H}. {T}. and {B}ouvier, {T}.}, editor = {}, language = {{ENG}}, abstract = {{U}sing three different microscopy techniques (epifluorescence, electronic and atomic force microscopy), we showed that high-{M}g calcite grains in calcifying microbial mats from the hypersaline lake "{L}a {S}alada de {C}hiprana", {S}pain, contain viruses with a diameter of 50-80 nm. {E}nergy-dispersive {X}-ray spectrometer analysis revealed that they contain nitrogen and phosphorus in a molar ratio of similar to 9, which is typical for viruses. {N}ucleic acid staining revealed that they contain {DNA} or {RNA}. {A}s characteristic for hypersaline environments, the concentrations of free and attached viruses were high (>10(10) viruses per g of mat). {I}n addition, we showed that acid treatment (dissolution of calcite) resulted in release of viruses into suspension and estimated that there were similar to 15 x 109 viruses per g of calcite. {W}e suggest that virus-mineral interactions are one of the possible ways for the formation of nano-sized structures often described as "nanobacteria" and that viruses may play a role in initiating calcification.}, keywords = {{ESPAGNE}}, booktitle = {}, journal = {{P}los {O}ne}, volume = {10}, numero = {6}, pages = {e0130552 [19 p.]}, ISSN = {1932-6203}, year = {2015}, DOI = {10.1371/journal.pone.0130552}, URL = {https://www.documentation.ird.fr/hor/fdi:010064852}, }