@article{fdi:010064850,
  title = {{M}ultilocus microsatellite typing of {L}eishmania infantum isolates in monitored {L}eishmania/{HIV} coinfected patients},
  author = {{T}omas-{P}erez, {M}. and {H}ide, {M}. and {R}iera, {C}. and {M}ontoya, {L}. and {B}anuls, {A}nne-{L}aure and {R}ibera, {E}. and {P}ortus, {M}. and {F}isa, {R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {L}eishmania infantum is the main etiological agent of both visceral and cutaneous clinical forms of leishmaniasis in the {M}editerranean area. {L}eishmania/{HIV} coinfection in this area is characterized by a chronic course and frequent recurrences of clinical episodes. {T}he present study using {M}ultilocus {M}icrosatellite {T}yping ({MLMT}) analysis, a highly discriminative tool, aimed to genetically characterize {L}. infantum isolates taken from monitored {L}eishmania/{HIV} coinfected patients presenting successive clinical episodes. {M}ethods: {I}n this study, by the analysis of 20 microsatellite loci, we studied the {MLMT} profiles of 25 {L}. infantum isolates from 8 {L}eishmania/{HIV} coinfected patients who had experienced several clinical episodes. {T}wo to seven isolates per patient were taken before and after treatment, during clinical and non-clinical episodes, with time intervals of 6 days to 29 months. {G}enetic diversity, clustering and phenetic analyses were performed. {R}esults: {MLMT} enabled us to study the genetic characteristics of the 25 {L}. infantum isolates, differentiating 18 genotypes, corresponding to a genotypic diversity of 0.72. {F}ifteen genotypes were unique in the total sample set and only 3 were repeated, 2 of which were detected in different patients. {B}oth clustering and phylogenetic analyses provided insights into the genetic links between the isolates; in five patients isolates showed clear genetic links: either the genotype was exactly the same or only slightly different. {I}n contrast, the isolates of the other three patients were dispersed in different clusters and some could be the result of mixing between populations. {C}onclusions: {O}ur data indicated a great {MLMT} variability between isolates from coinfected patients and no predominant genotype was observed. {D}espite this, almost all clinical episodes could be interpreted as a relapse rather than a reinfection. {T}he results showed that diverse factors like an intrapatient evolution over time or culture bias could influence the parasite population detected in the patient, making it difficult to differentiate between relapse and reinfection.},
  keywords = {{L}eishmania infantum ; {HIV} ; {M}icrosatellites ; {G}enetic diversity ; {P}henetic analyses ; {R}elapse ; {R}einfection ; {ESPAGNE} ; {ZONE} {MEDITERRANEENNE}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {8},
  numero = {},
  pages = {art. 386 [9 ]},
  ISSN = {1756-3305},
  year = {2015},
  DOI = {10.1186/s13071-015-0989-9},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064850},
}