Phommasone K., Sengvilaipaseuth O., de Lamballerie Xavier, Vongsouvath M., Phonemixay O., Blacksell S. D., Newton P. N., Dubot Pérès Audrey. (2015). Temperature and the field stability of a dengue rapid diagnostic test in the tropics. American Journal of Tropical Medicine and Hygiene, 93 (1), p. 33-39. ISSN 0002-9637.
Titre du document
Temperature and the field stability of a dengue rapid diagnostic test in the tropics
Année de publication
2015
Auteurs
Phommasone K., Sengvilaipaseuth O., de Lamballerie Xavier, Vongsouvath M., Phonemixay O., Blacksell S. D., Newton P. N., Dubot Pérès Audrey
Source
American Journal of Tropical Medicine and Hygiene, 2015,
93 (1), p. 33-39 ISSN 0002-9637
The global incidence of dengue has increased significantly in recent decades, resulting in a large public health burden in tropical and subtropical countries. Dengue rapid diagnostic tests (RDTs) can provide accurate, rapid accessible diagnosis for patient management and may be easily used by health workers in rural areas. However, in dengue-endemic areas, ambient temperatures are often higher than manufacturer's recommendation. We therefore evaluated the effect of high temperature over time on the performance of one commonly used dengue RDT, the Standard Diagnostics Bioline Dengue Duo. RDTs were kept in five different conditions (at 4 degrees C, 35 degrees C, 45 degrees C, 60 degrees C, and at fluctuant ambient temperatures in a free-standing hut) for between 2 days and 2 years in the Lao People's Democratic Republic (PDR). RDTs were tested with four control sera (negative, dengue nonstructural protein 1 [NS1], anti-dengue immunoglobulin [Ig] M, and anti-dengue IgG positive). The RDTs had 100% consistency over the 2-year study, despite high temperatures, including in the hut in which temperatures exceeded the manufacturer's recommendations for 29% of time points. These data suggest that the diagnostic accuracy of the SD Bioline Dengue Duo RDT remains stable even after long-term storage at high temperatures. Therefore, use at such ambient temperatures in tropical areas should not jeopardize the dengue diagnostic outcome.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
LAOS ; ZONE TROPICALE
Localisation
Fonds IRD [F B010064813]
Identifiant IRD
fdi:010064813
