@article{fdi:010064777,
  title = {{E}vidence of two mechanisms involved in {B}acillus thuringiensis israelensis decreased toxicity against mosquito larvae : genome dynamic and toxins stability},
  author = {{E}lleuch, {J}. and {Z}ghal, {R}. {Z}. and {L}acroix, {M}arie-{N}o{\¨e}lle and {C}handre, {F}abrice and {T}ounsi, {S}. and {J}aoua, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}iopesticides based on {B}acillus thuringiensis israelensis are the most used and most successful around the world. {T}his bacterium is characterized by a dynamic genome able to win or lose genetic materials which leads to a decrease in its effectiveness. {T}he detection of such phenomena is of great importance to monitor the stability of {B}. thuringiensis strains in industrial production processes of biopesticides. {N}ew local {B}. thuringiensis israelensis isolates were investigated. {T}hey present variable levels of delta-endotoxins production and insecticidal activities against {A}edes aegypti larvae. {S}earching on the origin of this variability, molecular and biochemical analyses were performed. {T}he obtained results describe two main reasons of the decrease of {B}. thuringiensis israelensis insecticidal activity. {T}he first reason was the deletion of cry4{A}a and cry10{A}a genes from the 128-kb p{B}toxis plasmid as evidenced in three strains ({BLB}124, {BLB}199 and {BLB}506) among five. {T}he second was the early degradation of {C}ry toxins by proteases in larvae midgut mainly due to some amino acids substitutions evidenced in {C}ry4{B}a and {C}ryl1{A}a delta-endotoxins detected in {BLB}356. {B}efore biological treatment based on {B}. thuringiensis israelensis, the studies of microflore in each ecosystem have a great importance to succeed pest management programs.},
  keywords = {{B}acillus thuringiensis israelensis ; {A}edes aegypti ; cry genes content ; {P}roteolysis},
  booktitle = {},
  journal = {{M}icrobiological {R}esearch},
  volume = {176},
  numero = {},
  pages = {48--54},
  ISSN = {0944-5013},
  year = {2015},
  DOI = {10.1016/j.micres.2015.04.007},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064777},
}