%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Cejas, I.
%A Rivas, M.
%A Napoles, L.
%A Marrero, P.
%A Yabor, L.
%A Aragon, C.
%A Perez, A.
%A Engelmann, Florent
%A Martinez-Montero, M. E.
%A Lorenzo, J. C.
%T Impact of liquid nitrogen exposure on selected biochemical and structural parameters of hydrated Phaseolus vulgaris L. seeds
%D 2015
%L fdi:010064736
%G ENG
%J Cryoletters
%@ 0143-2044
%K Common bean ; cryopreservation ; cryostorage
%M ISI:000357552300001
%N 3
%P 149-157
%U https://www.documentation.ird.fr/hor/fdi:010064736
%> https://www.documentation.ird.fr/intranet/publi/2015/07/010064736.pdf
%V 36
%W Horizon (IRD)
%X BACKGROUND: It is well known that cryopreserving seeds with high water content is detrimental to survival, but biochemical and structural parameters of cryostored hydrated common bean seeds have not been published. OBJECTIVE: The objective of this work was to study the effect of liquid nitrogen exposure on selected biochemical and structural parameters of hydrated Phaseolus vulgaris seeds. MATERIALS AND METHODS: We cryopreserved seeds at various moisture contents and evaluated: germination; electrolyte leakage; fresh seed weight; levels of chlorophyll pigments, malondialdehyde, other aldehydes, phenolics and proteins; thickness of cotyledon epidermis, parenchyma, and starch storage parenchyma; and radicle and plumule lengths. RESULTS: Germination was totally inhibited when seeds were immersed in water for 50 min (moisture content of 38%, FW basis) before cryopreservation. The combined effects of seed water imbibition and cryostorage decreased phenolics (free, cell wall-linked, total), chlorophyll a and protein content. By contrast, electrolyte leakage and levels of chlorophyll b and other aldehydes increased as a result of the combination of these two experimental factors. These were the most significant effects observed during exposure of humid seed to liquid nitrogen. CONCLUSION: Further studies are still required to clarify the molecular events taking place in plant cells during eryostorage.
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