@article{fdi:010064673,
  title = {{D}ifferential expression of midgut proteins in {T}rypanosoma brucei gambiense-stimulated vs. non-stimulated {G}lossina palpalis gambiensis flies},
  author = {{G}eiger, {A}nne and {S}oumana, {I}. {H}. and {T}chicaya, {B}. and {R}ofidal, {V}. and {D}ecourcelle, {M}. and {S}antoni, {V}. and {H}em, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he unicellular pathogenic protozoan {T}rypanosoma brucei gambiense is responsible for the chronic form of sleeping sickness. {T}his vector-borne disease is transmitted to humans by the tsetse fly of the group {G}lossina palpalis, including the subspecies {G}. p. gambiensis, in which the parasite completes its developmental cycle. {S}leeping sickness control strategies can therefore target either the human host or the fly vector. {I}ndeed, suppression of one step in the parasite developmental cycle could abolish parasite transmission to humans, with consequences on the spreading of the disease. {I}n order to develop this type of approach, we have identified, at the proteome level, events resulting from the tripartite interaction between the tsetse fly {G}. p. gambiensis, its microbiome, and the trypanosome. {P}roteomes were analyzed from four biological replicates of midguts from flies sampled 3 days post-feeding on either a trypanosome-infected (stimulated flies) or a non-infected (non-stimulated flies) bloodmeal. {O}ver 500 proteins were identified in the midguts of flies from both feeding groups, 13 of which were shown to be differentially expressed in trypanosome-stimulated vs. non-stimulated flies. {F}unctional annotation revealed that several of these proteins have important functions that could be involved in modulating the fly infection process by trypanosomes (and thus fly vector competence), including anti-oxidant and anti-apoptotic, cellular detoxifying, trypanosome agglutination, and immune stimulating or depressive effects. {T}he results show a strong potential for diminishing or even disrupting fly vector competence, and their application holds great promise for improving the control of sleeping sickness.},
  keywords = {sleeping sickness ; tsetse-bacteria-trypanosomes ; tripartite interactions ; trypanosome-associated global changes ; label-free quantification},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {6},
  numero = {},
  pages = {art. 444 [12 p.]},
  ISSN = {1664-302{X}},
  year = {2015},
  DOI = {10.3389/fmicb.2015.00444},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064673},
}