@article{fdi:010064617,
  title = {{R}eversible oxygen-tolerant hydrogenase carried by free-living {N}-2-fixing bacteria isolated from the rhizospheres of rice, maize, and wheat},
  author = {{R}oumagnac, {P}. and {R}ichaud, {P}. and {B}arakat, {M}. and {O}rtet, {P}. and {R}oncato, {M}. {A}. and {H}eulin, {T}. and {P}eltier, {G}. and {A}chouak, {W}. and {C}ournac, {L}aurent},
  editor = {},
  language = {{ENG}},
  abstract = {{H}ydrogen production by microorganisms is often described as a promising sustainable and clean energy source, but still faces several obstacles, which prevent practical application. {A}mong them, oxygen sensitivity of hydrogenases represents one of the major limitations hampering the biotechnological implementation of photobiological production processes. {H}ere, we describe a hierarchical biodiversity-based approach, including a chemochromic screening of hydrogenase activity of hundreds of bacterial strains collected from several ecosystems, followed by mass spectrometry measurements of hydrogenase activity of a selection of the {H}-2-oxidizing bacterial strains identified during the screen. {I}n all, 131 of 1266 strains, isolated from cereal rhizospheres and basins containing irradiating waste, were scored as {H}-2-oxidizing bacteria, including {P}seudomonas sp., {S}erratia sp., {S}tenotrophomonas sp., {E}nterobacter sp., {R}ahnella sp., {B}urkholderia sp., and {R}alstonia sp. isolates. {F}our free-living {N}-2-fixing bacteria harbored a high and oxygen-tolerant hydrogenase activity, which was not fully inhibited within entire cells up to 150-250 mu mol/{L} {O}-2 concentration or within soluble protein extracts up to 25-30 mu mol/{L}. {T}he only hydrogenase-related genes that we could reveal in these strains were of the hyc type (subunits of formate hydrogenlyase complex). {T}he four free-living {N}-2-fixing bacteria were closely related to {E}nterobacter radicincitans based on the sequences of four genes (16{S} r{RNA}, rpo{B}, hsp60, and hyc{E} genes). {T}hese results should bring interesting prospects for microbial biohydrogen production and might have ecophysiological significance for bacterial adaptation to the oxic-anoxic interfaces in the rhizosphere.},
  keywords = {{D}iversity ; engineering ; hydrogen metabolism ; hydrogenase ; oxygen ; {FRANCE} ; {EGYPTE} ; {SENEGAL} ; {MARTINIQUE} ; {VIETNAM}},
  booktitle = {},
  journal = {{M}icrobiologyopen},
  volume = {1},
  numero = {4},
  pages = {349--361},
  ISSN = {2045-8827},
  year = {2012},
  DOI = {10.1002/mbo3.37},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064617},
}