@article{fdi:010064610,
  title = {{C}omparative proteomics reveal new {H}rp{X}-regulated proteins of {X}anthomonas oryzae pv. oryzae},
  author = {{R}obin, {G}. {P}. and {O}rtiz, {E}. and {S}zurek, {B}oris and {B}rizard, {J}ean-{P}aul and {K}oebnik, {R}alf},
  editor = {},
  language = {{ENG}},
  abstract = {{P}athogenicity of the rice pathogenic bacterium {X}anthomonas oryzae pv. oryzae depends on a {H}rp (hypersensitive response and pathogenicity) type {III} secretion system; the expression of which is induced in planta. {E}xpression of the hrp operons is under transcriptional control of two key regulatory proteins, {H}rp{G} and {H}rp{X}. {T}o identify new proteins that are co-regulated with the type {III} secretion system, we employed comparative proteomics. {C}ells of {X}. oryzae pv. oryzae ectopically expressing hrp{X} were compared to wild-type cells grown in vitro. {T}wenty protein spots with different abundances in both samples were identified by 2{D}-{DIGE} and {LC}-{MS}/{MS}. {S}even spots could be unambiguously identified, corresponding to the {H}rp{B}1 protein, two different peptidyl-prolyl cis-trans isomerases, a component of an {ATP} binding cassette ({ABC}) transport system, an adenylate kinase, and a secreted protein of unknown function. {I}nterestingly, the isoelectric point of the adenylate kinase was found to be under control of {H}rp{X}, most likely due to post-translational modification. {I}ndeed, two glutamate residues of the adenylate kinase were found to be methylated but this modification did not account for the shift in electrophoretic mobility. {I}n summary, we identified new {H}rp{X}-regulated proteins of {X}. oryzae pv. oryzae that might be important for pathogenicity. {T}his article is part of a {S}pecial {I}ssue entitled: {T}rends in microbial proteomics. {B}iological significance {W}e use 2{D}-{DIGE} to compare the proteomes of rice-pathogenic xanthomonads. {W}e identify seven proteins that are co-regulated with the type {III} secretion system. {W}e find posttranslational glutamate methylation of a bacterial adenylate cyclase. {T}he newly identified {H}rp{X}-regulated proteins might be important for pathogenicity.},
  keywords = {{C}omparative proteomics ; 2{D}-{DIGE} ; {X}anthomonas oryzae ; {H}rp{X} regulon ; {A}denylate lcinase ; {G}lutamate methylation},
  booktitle = {},
  journal = {{J}ournal of {P}roteomics},
  volume = {97},
  numero = {},
  pages = {256--264},
  ISSN = {1874-3919},
  year = {2014},
  DOI = {10.1016/j.jprot.2013.04.010},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064610},
}