Mathis A., Depaquit J., Dvorak V., Tuten H., Banuls Anne-Laure, Halada P., Zapata S., Lehrter V., Hlavackova K., Prudhomme Jorian, Volf P., Sereno Denis, Kaufmann C., Pfluger V., Schaffner F. (2015). Identification of phlebotomine sand flies using one MALDI-TOF MS reference database and two mass spectrometer systems. Parasites and Vectors, 8, p. 266. ISSN 1756-3305.
Titre du document
Identification of phlebotomine sand flies using one MALDI-TOF MS reference database and two mass spectrometer systems
Année de publication
2015
Auteurs
Mathis A., Depaquit J., Dvorak V., Tuten H., Banuls Anne-Laure, Halada P., Zapata S., Lehrter V., Hlavackova K., Prudhomme Jorian, Volf P., Sereno Denis, Kaufmann C., Pfluger V., Schaffner F.
Source
Parasites and Vectors, 2015,
8, p. 266 ISSN 1756-3305
Background: Rapid, accurate and high-throughput identification of vector arthropods is of paramount importance in surveillance programmes that are becoming more common due to the changing geographic occurrence and extent of many arthropod-borne diseases. Protein profiling by MALDI-TOF mass spectrometry fulfils these requirements for identification, and reference databases have recently been established for several vector taxa, mostly with specimens from laboratory colonies. Methods: We established and validated a reference database containing 20 phlebotomine sand fly (Diptera: Psychodidae, Phlebotominae) species by using specimens from colonies or field-collections that had been stored for various periods of time. Results: Identical biomarker mass patterns ('superspectra') were obtained with colony-or field-derived specimens of the same species. In the validation study, high quality spectra (i.e. more than 30 evaluable masses) were obtained with all fresh insects from colonies, and with 55/59 insects deep-frozen (liquid nitrogen/-80 degrees C) for up to 25 years. In contrast, only 36/52 specimens stored in ethanol could be identified. This resulted in an overall sensitivity of 87 % (140/161); specificity was 100 %. Duration of storage impaired data counts in the high mass range, and thus cluster analyses of closely related specimens might reflect their storage conditions rather than phenotypic distinctness. A major drawback of MALDI-TOF MS is the restricted availability of in-house databases and the fact that mass spectrometers from 2 companies (Bruker, Shimadzu) are widely being used. We have analysed fingerprints of phlebotomine sand flies obtained by automatic routine procedure on a Bruker instrument by using our database and the software established on a Shimadzu system. The sensitivity with 312 specimens from 8 sand fly species from laboratory colonies when evaluating only high quality spectra was 98.3 %; the specificity was 100 %. The corresponding diagnostic values with 55 field-collected specimens from 4 species were 94.7 % and 97.4 %, respectively. Conclusions: A centralized high-quality database (created by expert taxonomists and experienced users of mass spectrometers) that is easily amenable to customer-oriented identification services is a highly desirable resource. As shown in the present work, spectra obtained from different specimens with different instruments can be analysed using a centralized database, which should be available in the near future via an online platform in a cost-efficient manner.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Entomologie médicale / Parasitologie / Virologie [052]
Localisation
Fonds IRD [F B010064217]
Identifiant IRD
fdi:010064217
