@article{fdi:010064200,
  title = {{A}ntiproliferative activity and phenotypic modification induced by selected {P}eruvian medicinal plants on human hepatocellular carcinoma {H}ep3{B} cells},
  author = {{C}arraz, {M}a{\¨e}lle and {L}avergne, {C}. and {J}ullian, {V}al{\'e}rie and {W}right, {M}. and {G}airin, {J}. {E}. and de la {C}ruz, {M}. {G}. and {B}ourdy, {G}enevi{\`e}ve},
  editor = {},
  language = {{ENG}},
  abstract = {{E}thnopharmacological relevance: {T}he high incidence of human hepatocellular carcinoma ({HCC}) in {P}eru and the wide use of medicinal plants in this country led us to study the activity against {HCC} cells in vitro of somes species used locally against liver and digestive disorders. {M}aterials and methods: {E}thnopharmacological survey: {M}edicinal plant species with a strong convergence of use for liver and digestive diseases were collected fresh in the wild or on markets, in two places of {P}eru: {C}hiclayo ({L}ambayeque department, {C}hiclayo province) and {H}uaraz ({A}ncash department, {H}uaraz province). {A}ltogether 51 species were collected and 61 ethanol extracts were prepared to be tested. {B}iological assessment: {A}ll extracts were first assessed against the {HCC} cell line {H}ep3{B} according a 3-step multi-parametric phenotypic assay. {I}t included 1) the evaluation of phenotypic changes on cells by light microscopy, 2) the measurement of the antiproliferative activity and 3) the analysis of the cytoskeleton and mitosis by immunofluorescence. {B}est extracts were further assessed against other {HCC} cell lines {H}ep{G}2, {PLC}/{PRF}/5 and {SNU}-182 and their toxicity measured in vitro on primary human hepatocytes. {R}esults: {E}thnopharmacological survey: {S}ome of the species collected had a high reputation spreading over the surveyed locations for treating liver problems, {L}e. {B}accharis genistelloides, {B}ejaria aestuans, {C}entaurium pulchellum, {D}esmodium molliculum, {D}ipsacus fullonum, {E}quisetum bogotense, {G}entianella spp., {K}rameria lapacea, {O}tholobium spp., {S}chkuhria pinnata, {T}araxacum officinale. {H}ep3{B} evaluation: {F}ourteen extracts from 13 species ({A}chyrocline alata, {A}mbrosia arborescens, {B}accharis latifolia, {H}ypericum laricifolium, {K}rameria lappacea, {N}iphidium crassifolium, {O}phryosporus chilca, {O}rthrosanthus chimboracensis, {O}tholobium pubescens, {P}assiflora ligularis, {P}erezia coerulescens, {P}erezia multiflora and {S}chkuhria pinnata) showed a significant antiproliferative activity against {H}ep3{B} cells ({IC}50 <= 50 mu g/m{L}). {T}his was associated with a lack of toxicity on primary human hepatocytes in vitro. {I}mmunofluorescence experiments on {H}ep3{B} cells showed that crude extracts of {S}chkuhria pinnata and {O}rthrosanthus chimboracensis could block {H}ep3{B} cells in mitosis with an original phenotype. {C}rude extracts of {P}erezia coerulescens, {P}erezia multiflora, {A}chyrocline alata, {O}phryosporus chilca, {O}tholobium pubescens and {H}ypericum laricifolium could modify the overall microtubule cytoskeletal dynamics of {H}ep3{B} cells in interphase by an original mechanism. {C}onclusions: {O}ur method allowed us to select 9 extracts which displayed antiproliferative activities associated with original cellular phenotypes on {H}ep3{B} cells, regarding known microtubule-targeting drugs. {B}oth chemical and cellular studies are ongoing in order to elucidate natural compounds and cellular mechanisms responsible of the activities described.},
  keywords = {{P}eru ; {M}edicinal plants ; {H}epatocellular carcinoma ; {H}ep 3{B} cell line ; {L}iver ; {P}henotypic cell based ; {A}ssay ; {PEROU}},
  booktitle = {},
  journal = {{J}ournal of {E}thnopharmacology},
  volume = {166},
  numero = {},
  pages = {185--199},
  ISSN = {0378-8741},
  year = {2015},
  DOI = {10.1016/j.jep.2015.02.028},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064200},
}