@article{fdi:010064195,
  title = {{E}ffect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses},
  author = {{P}astorino, {B}. and {B}aronti, {C}{\'e}cile and {G}ould, {E}. {A}. and {C}harrel, {R}. {N}. and de {L}amballerie, {X}avier},
  editor = {},
  language = {{ENG}},
  abstract = {{A}s a partner of the {E}uropean {V}irus {A}rchive ({EVA}) {FP}7 project, our laboratory maintains a large collection of freeze-dried viruses. {T}he distribution of these viruses to academic researchers, public health organizations and industry is one major aim of the {EVA} consortium. {I}t is known that lyophilization requires appropriate stabilizers to prevent inactivation of the virus. {H}owever, few studies have investigated the influence of different stabilizers and lyophilization protocols on the thermostability of different viruses. {I}n order to identify optimal lyophilization conditions that will deliver maximum retention of viral infectivity titre, different stabilizer formulations containing trehalose, sorbitol, sucrose or foetal bovine serum were evaluated for their efficacy in stabilizing a representative panel of freeze dried viruses at different storage temperatures (-20 degrees {C}, +4 degrees {C} and +20 degrees {C}) for one week, the two latter mimicking suboptimal shipping conditions. {T}he {T}issue {C}ulture {I}nfectious {D}ose 50% ({TCID}50) assay was used to compare the titres of infectious virus. {T}he results obtained using four relevant and model viruses (enveloped/non enveloped {RNA}/{DNA} viruses) still serve to improve the freeze drying conditions needed for the development and the distribution of a large virus collection.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {10},
  numero = {4},
  pages = {e0118963 [12 p.]},
  ISSN = {1932-6203},
  year = {2015},
  DOI = {10.1371/journal.pone.0118963},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064195},
}