Publications des scientifiques de l'IRD

Pastorino B., Baronti Cécile, Gould E. A., Charrel R. N., de Lamballerie Xavier. (2015). Effect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses. Plos One, 10 (4), p. e0118963 [12 p.]. ISSN 1932-6203.

Titre du document
Effect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000353711600002
Auteurs
Pastorino B., Baronti Cécile, Gould E. A., Charrel R. N., de Lamballerie Xavier
Source
Plos One, 2015, 10 (4), p. e0118963 [12 p.] ISSN 1932-6203
As a partner of the European Virus Archive (EVA) FP7 project, our laboratory maintains a large collection of freeze-dried viruses. The distribution of these viruses to academic researchers, public health organizations and industry is one major aim of the EVA consortium. It is known that lyophilization requires appropriate stabilizers to prevent inactivation of the virus. However, few studies have investigated the influence of different stabilizers and lyophilization protocols on the thermostability of different viruses. In order to identify optimal lyophilization conditions that will deliver maximum retention of viral infectivity titre, different stabilizer formulations containing trehalose, sorbitol, sucrose or foetal bovine serum were evaluated for their efficacy in stabilizing a representative panel of freeze dried viruses at different storage temperatures (-20 degrees C, +4 degrees C and +20 degrees C) for one week, the two latter mimicking suboptimal shipping conditions. The Tissue Culture Infectious Dose 50% (TCID50) assay was used to compare the titres of infectious virus. The results obtained using four relevant and model viruses (enveloped/non enveloped RNA/DNA viruses) still serve to improve the freeze drying conditions needed for the development and the distribution of a large virus collection.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Entomologie médicale / Parasitologie / Virologie [052] ; Biotechnologies [084]
Localisation
Fonds IRD [F B010064195]
Identifiant IRD
fdi:010064195
Contact