@article{fdi:010064191,
  title = {{A}ntiplasmodial and anti-inflammatory effects of an antimalarial remedy from the {W}ayana {A}merindians, {F}rench {G}uiana : takamalaime ({P}sidium acutangulum {M}art. ex {DC}., {M}yrtaceae)},
  author = {{H}ouel, {E}. and {F}leury, {M}arie and {O}donne, {G}. and {N}ardella, {F}. and {B}ourdy, {G}enevi{\`e}ve and {V}onthron-{S}enecheau, {C}. and {V}illa, {P}. and {O}brecht, {A}. and {E}parvier, {V}. and {D}eharo, {E}ric and {S}tien, {D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{E}thnopharmacological relevance: {F}ield investigations highlighted the use of {P}sidium acutangulum {M}art. ex {DC} (syn. {P}. persoonii {M}c{V}augh), a small tree used by the {W}ayana {A}merindians in {T}wenke-{T}aluhwen and {A}ntecume-{P}ata, {F}rench {G}uiana, for the treatment of malaria, and administered either orally in the form of a decoction or applied externally over the whole body. {T}his use appears limited to the {W}ayana cultural group in {F}rench {G}uiana and has never been reported anywhere else. {O}ur goal was to evaluate the antimalarial and anti-inflammatory activities of a {P}. acutangulum decoction to explain the good reputation of this remedy. {M}aterials and methods: {I}nterviews with the {W}ayana inhabitants of {T}wenke-{T}aluhwen and {A}ntecume-{P}ata were conducted within the {TRAMAZ} project according to the {TRAMIL} methodology, which is based on a quantitative and qualitative analysis of medicinal plant uses. {A} decoction of dried aerial parts of {P}. acutangulum was prepared in consistency with the {W}ayana recipe. {I}n vitro antiplasmodial assays were performed on chloroquine-resistant {F}c{B}1 ([{H}-3]-hypoxanthine bioassay) and 7{G}8 (p{LDH} bioassay) {P}. falciparum strains and on chloroquine sensitive {NF}54 ([{H}-3]-hypoxanthine bioassay) {P}. falciparum strain. {I}n vitro anti-inflammatory activity ({IL}-1 beta, {IL}-6, {IL}-8, {TNF} alpha) was evaluated on {LPS}-stimulated human {PBMC} and {NO} secretion inhibition was measured on {LPS} stimulated {RAW} murine macrophages. {C}ytotoxicity of the decoction was measured on {L}6 mammalian cells, {PBMC}s, and {RAW} cells. {A} preliminary evaluation of the in vivo antimalarial activity of the decoction, administered orally twice daily, was assessed by the classical four-day suppressive test against {R} berghei {NK}65 in mice. {R}esults: {T}he decoction displayed a good antiplasmodial activity in vitro against the three tested strains, regardless to the bioassay used, with {IC}50 values of 3.3 mu g/m{L} and 10.3 mu g/m{L} against {P}. falciparum {F}c{B}1 and {NF}54, respectively and 19.0 mu g/{ML}. against {P}. falciparum 7{G}8. {I}t also exhibited significant anti-inflammatory activity in vitro in a dose dependent manner. {A}t a concentration of 50 mu g/m{L}, the decoction inhibited the secretion of the following pro-inflammatory cytokines: {TNF} alpha (-18%), {IL}-1 beta (-58%), {IL}-6 (-32%), {IL}-8 (-21%). {I}t also exhibited a mild {NO} secretion inhibition (-13%) at the same concentration. {T}he decoction was non-cytotoxic against {L}6 cells ({IC}50 > 100 mu g/m{L}), {RAW} cells and {PBMC}. {I}n vivo, 150 mu {L}. of the decoction given orally twice a day (equivalent to 350 mg/kg/day of dried extract) inhibited 39.7% average parasite growth, with more than 50% of inhibition in three mice over five. {T}he absence of response for the two remaining mice, however, induced a strong standard deviation. {C}onclusions: {T}his study highlighted the in vitro antiplasmodial activity of the decoction of {P}. acutangulum aerial parts, used by {W}ayana {A}merindians from the {U}pper-{M}aroni in {F}rench {G}uiana in case of malaria. {I}ts antioxidant and anti-inflammatory potential, which may help to explain its use against this disease, was demonstrated using models of artificially stimulated cells.},
  keywords = {{P}sidium acutangulum ; {P}lasmodium ; {C}ytokines ; {A}ntimalarial ; {F}rench {G}uiana ; {T}raditional medicine ; {GUYANE} {FRANCAISE}},
  booktitle = {},
  journal = {{J}ournal of {E}thnopharmacology},
  volume = {166},
  numero = {},
  pages = {279--285},
  ISSN = {0378-8741},
  year = {2015},
  DOI = {10.1016/j.jep.2015.03.015},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064191},
}