@article{fdi:010064186,
  title = {{E}valuation of molecular methods to improve the detection of {B}urkholderia pseudomallei in soil and water samples from {L}aos},
  author = {{K}nappik, {M}. and {D}ance, {D}. {A}. {B}. and {R}attanavong, {S}. and {P}ierret, {A}lain and {R}ibolzi, {O}livier and {D}avong, {V}. and {S}ilisouk, {J}. and {V}ongsouvath, {M}. and {N}ewton, {P}. {N}. and {D}ittrich, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}urkholderia pseudomallei is the cause of melioidosis, a severe and potentially fatal disease of humans and animals. {I}t is endemic in northern {A}ustralia and {S}outheast {A}sia and is found in soil and surface water. {T}he environmental distribution of {B}. pseudomallei worldwide and within countries where it is endemic, such as the {L}ao {P}eople's {D}emocratic {R}epublic ({L}aos), remains unclear. {H}owever, this knowledge is important to our understanding of the ecology and epidemiology of {B}. pseudomallei and to facilitate public health interventions. {S}ensitive and specific methods to detect {B}. pseudomallei in environmental samples are therefore needed. {T}he aim of this study was to compare molecular and culture-based methods for the detection of {B}. pseudomallei in soil and surface water in order to identify the optimal approach for future environmental studies in {L}aos. {M}olecular detection by quantitative real-time {PCR} (q{PCR}) was attempted after {DNA} extraction directly from soil or water samples or after an overnight enrichment step. {T}he positivity rates obtained by q{PCR} were compared to those obtained by different culture techniques. {T}he rate of detection from soil samples by q{PCR} following culture enrichment was significantly higher (84/100) than that by individual culture methods and all culture methods combined (44/100; {P} < 0.001). {S}imilarly, q{PCR} following enrichment was the most sensitive method for filtered river water compared with the sensitivity of the individual methods and all individual methods combined. {I}n conclusion, molecular detection following an enrichment step has proven to be a sensitive and reliable approach for {B}. pseudomallei detection in {L}ao environmental samples and is recommended as the preferred method for future surveys.},
  keywords = {{LAOS}},
  booktitle = {},
  journal = {{A}pplied and {E}nvironmental {M}icrobiology},
  volume = {81},
  numero = {11},
  pages = {3722--3727},
  ISSN = {0099-2240},
  year = {2015},
  DOI = {10.1128/aem.04204-14},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064186},
}