@article{fdi:010064182,
  title = {{E}xperimental evidence of complex relationships between the ambient salinity and the strontium signature of fish otoliths},
  author = {{P}anfili, {J}acques and {D}arnaude, {A}. {M}. and {V}igliola, {L}aurent and {J}acquart, {A}. and {L}abonne, {M}aylis and {G}illes, {S}ylvain},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he otolith strontium:calcium ratio ({S}r:{C}a) has been widely used to assess the connectivity between fish populations in ocean, estuarine and freshwater environments as the concentration of {S}r in the otoliths is strongly correlated with water salinity. {T}his correlation was tested experimentally in hypersaline conditions by submitting the extremely euryhaline tilapia species {S}arotherodon melanotheron heudelotii ({C}ichlidae), found throughout {W}est {A}frican continental waters and commonly used as an aquaculture species, to a strong salinity gradient (15-106). {E}xperimental and control individuals were reared from birth in a closed system at a constant salinity of 10 and injected with oxytetracycline ({OTC}) to mark the otoliths at the beginning of the experiment. {R}andomly selected control fish were maintained for 75 days at salinities of 10-20. {T}he remaining experimental fish were acclimated to a salinity above 100 which was reduced by 10 each week to a salinity of 20. {T}he salinity and temperature of the water were recorded every day and the {S}r concentrations in the water were measured weekly by solution-based {ICP}-{MS}. {T}he fish from the control and experimental groups were sampled weekly and otolith transverse sections were prepared for {S}r:{C}a measurements by laser ablation {ICP}-{MS}. {N}o significant difference in the otolith growth rates after {OTC} marking was found between the control and experimental groups ({ANCOVA}, p = 0.63), showing that the experimental design did not affect fish growth. {T}he {S}r concentration in the water was closely related to ambient salinity (positive linear regression, {R}-2 = 0.96). {F}or most of the fish tested (similar to 80%), the relationship between otolith {S}r:{C}a and salinity was positive but nonlinear (power law, {R}-2 = 0.77 on log-log plot). {H}owever, about 20% of individuals from both the control and the experimental groups showed consistently low {S}r:{C}a ratios irrespective of the salinity, suggesting that the {S}r incorporation into the otoliths in these fish was strongly regulated. {T}his shows that there is high variability between individuals in the regulation of {S}r incorporation by a euryhaline species and indicates that otolith datasets for ecological applications should be interpreted with caution.},
  keywords = {{E}uryhaline tilapia ; {O}tolith microchemistry ; {S}alinity ; {S}arotherodon ; melanotheron heudelotii ; {S}trontium ; {V}alidation},
  booktitle = {},
  journal = {{J}ournal of {E}xperimental {M}arine {B}iology and {E}cology},
  volume = {467},
  numero = {},
  pages = {65--70},
  ISSN = {0022-0981},
  year = {2015},
  DOI = {10.1016/j.jembe.2015.03.007},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064182},
}