@article{fdi:010064171,
  title = {{P}lasmodium falciparum mating patterns and mosquito infectivity of natural isolates of gametocytes},
  author = {{M}orlais, {I}sabelle and {N}sango, {S}. {E}. and {T}oussile, {W}. and {A}bate, {L}uc and {A}nnan, {Z}. and {T}chioffo, {M}. {T}. and {C}ohuet, {A}nna and {A}wono-{A}mbene, {P}. {H}. and {F}ontenille, {D}idier and {R}ousset, {F}. and {B}erry, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}lasmodium falciparum infections in malaria endemic areas often harbor multiple clones of parasites. {H}owever, the transmission success of the different genotypes within the mosquito vector has remained elusive so far. {T}he genetic diversity of malaria parasites was measured by using microsatellite markers in gametocyte isolates from 125 asymptomatic carriers. {F}or a subset of 49 carriers, the dynamics of co-infecting genotypes was followed until their development within salivary glands. {A}lso, individual oocysts from midguts infected with blood from 9 donors were genotyped to assess mating patterns. {M}ultiplicity of infection ({MOI}) was high both in gametocyte isolates and sporozoite populations, reaching up to 10 genotypes. {G}ametocyte isolates with multiple genotypes gave rise to lower infection prevalence and intensity. {F}luctuations of genotype number occurred during the development within the mosquito and sub-patent genotypes, not detected in gametocyte isolates, were identified in the vector salivary glands. {T}he inbreeding coefficient {F}is was positively correlated to the oocyst loads, suggesting that {P}. falciparum parasites use different reproductive strategies according to the genotypes present in the gametocyte isolate. {T}he number of parasite clones within an infection affects the transmission success and the mosquito has an important role in maintaining {P}. falciparum genetic diversity. {O}ur results emphasize the crucial importance of discriminating between the different genotypes within an infection when studying the {A}. gambiae natural resistance to {P}. falciparum, and the need to monitor parasite diversity in areas where malaria control interventions are implemented.},
  keywords = {{CAMEROUN}},
  booktitle = {},
  journal = {{P}lo{S} {O}ne},
  volume = {10},
  numero = {4},
  pages = {e0123777 [14 ]},
  ISSN = {1932-6203},
  year = {2015},
  DOI = {10.1371/journal.pone.0123777},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064171},
}