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Ben Hania Wajdi, Joseph Manon, Fiebig A., Bunk B., Klenk H. P., Fardeau Marie-Laure, Spring S. (2015). Caldisalinibacter kiritimatiensis gen. nov., sp. nov., a moderately thermohalophilic thiosulfate-reducing bacterium from a hypersaline microbial mat. Geomicrobiology Journal, 32 (3-4), 347-354. ISSN 0149-0451

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Lien direct chez l'éditeur doi:10.1080/01490451.2013.866995

Titre
Caldisalinibacter kiritimatiensis gen. nov., sp. nov., a moderately thermohalophilic thiosulfate-reducing bacterium from a hypersaline microbial mat
Année de publication2015
Type de documentArticle référencé dans le Web of Science WOS:000352349600013
AuteursBen Hania Wajdi, Joseph Manon, Fiebig A., Bunk B., Klenk H. P., Fardeau Marie-Laure, Spring S.
SourceGeomicrobiology Journal, 2015, 32 (3-4), p. 347-354. ISSN 0149-0451
RésuméA novel anaerobic bacterium, designated L21-TH-D2(T) was isolated from the anoxic zone of a microbial mat of the hypersaline Lake 21 on the Kiritimati Atoll (Republic of Kiribati, Central Pacific). Cells were Gram-positive, spore-forming and motile slender rods. The novel strain grew anaerobically by fermentation at temperatures between 40 and 65 degrees C (optimum 50-55 degrees C) and at pH between 5-9 (optimum 6.9-7.0). It required at least 20g L-1 NaCl for growth and tolerated up to 150g L-1 NaCl (optimum at 50g L-1). In the presence of yeast extract or peptone the novel strain fermented glucose, fructose, maltose, mannose and pyruvate. The end-products from glucose fermentation were acetate, ethanol, CO2 and H-2. Nitrate, nitrite, elemental sulfur, sulfate, and sulfite were not used as electron acceptors, while thiosulfate was reduced to sulfide. The cellular fatty acids pattern was dominated by iso-C15:0 and iso-C15:0 DMA. The major polar lipids were identified as phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and two different unidentified glycolipids. No respiratory lipoquinones or cytochromes were detected. The DNA G + C content was 30.7mol%. The determined draft genome sequence of strain L21-TH-D2(T) revealed a specialization on a saccharolytic fermentative metabolism. Besides enzymes for substrate-level phosphorylation, several membrane-bound enzyme complexes, like an ion-translocating RNF complex and an F-type ATP synthase, were annotated in the genome sequence and seem to be involved in the energy metabolism. Phylogenetic analyses based on a comparison of 16S rRNA gene sequences placed the novel isolate in a clade of anaerobic halophilic or thermohalophilic bacteria within the clostridial subcluster XII. Significant phenotypic and phylogenetic differences prevent inclusion of the novel isolate in existing genera within the family Clostridiaceae, so that the novel taxon Caldisalinibacter kiritimatiensis gen. nov., sp. nov., is proposed. The type strain is L21-TH-D2(T) (= DSM 26826(T) = JCM 18664(T)).
Plan de classementBiotechnologies [084] ; Limnologie biologique / Océanographie biologique [034] ; Géologie et formations superficielles [064]
Descr. géo.PACIFIQUE ; MICRONESIE ; KIRIBATI ; KIRITIMATI ATOLL
LocalisationFonds IRD [F B010064161]
Identifiant IRDfdi:010064161
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010064161

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