%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Sylvestre, Isabelle
%A Engelmann, Florent
%T Cryopreservation of in vitro-grown shoot tips of Clinopodium odorum using aluminium cryo-plates
%D 2015
%L fdi:010064146
%G ENG
%J In Vitro Cellular & Developmental Biology-Plant
%@ 1054-5476
%K Cryopreservation ; Clinopodium odorum ; Shoot tips ; V cryo-plate ; D cryo-plate
%K ARGENTINE
%M ISI:000352920100008
%N 2
%P 185-191
%R 10.1007/s11627-015-9668-y
%U https://www.documentation.ird.fr/hor/fdi:010064146
%> https://www.documentation.ird.fr/intranet/publi/2015/05/010064146.pdf
%V 51
%W Horizon (IRD)
%X In this study, we applied the vitrification (V) cryo-plate protocol for cryopreservation of Clinopodium odorum in vitro-derived shoot tips. We studied the recovery of shoot tips after modification of various parameters of the V cryo-plate protocol, including sucrose concentration in the preconditioning medium, composition of the loading solution (LS) and vitrification solution (VS) and duration of treatment with PVS2 VS, which contained (w/v) 30% glycerol, 15% dimethylsulphoxide, 15% ethylene glycol and 13.7% sucrose. We also compared the efficiency of the V cryo-plate protocol with the dehydration (D) cryo-plate protocol. The optimal conditions determined for the V cryo-plate protocol included a 24-h preconditioning treatment on medium with 0.3 M sucrose; treatment for 20 min at room temperature with LS containing 2.0 M glycerol and 0.4 M sucrose; and dehydration with PVS2 for 60 min at 0A degrees C. Under these conditions, 71.0% recovery of cryopreserved shoot tips was achieved. Only 29.2% regeneration was noted with the D cryo-plate protocol.
%$ 076 ; 084