Publications des scientifiques de l'IRD

Sylvestre Isabelle, Engelmann Florent. (2015). Cryopreservation of in vitro-grown shoot tips of Clinopodium odorum using aluminium cryo-plates. In Vitro Cellular & Developmental Biology-Plant, 51 (2), p. 185-191. ISSN 1054-5476.

Titre du document
Cryopreservation of in vitro-grown shoot tips of Clinopodium odorum using aluminium cryo-plates
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000352920100008
Auteurs
Sylvestre Isabelle, Engelmann Florent
Source
In Vitro Cellular & Developmental Biology-Plant, 2015, 51 (2), p. 185-191 ISSN 1054-5476
In this study, we applied the vitrification (V) cryo-plate protocol for cryopreservation of Clinopodium odorum in vitro-derived shoot tips. We studied the recovery of shoot tips after modification of various parameters of the V cryo-plate protocol, including sucrose concentration in the preconditioning medium, composition of the loading solution (LS) and vitrification solution (VS) and duration of treatment with PVS2 VS, which contained (w/v) 30% glycerol, 15% dimethylsulphoxide, 15% ethylene glycol and 13.7% sucrose. We also compared the efficiency of the V cryo-plate protocol with the dehydration (D) cryo-plate protocol. The optimal conditions determined for the V cryo-plate protocol included a 24-h preconditioning treatment on medium with 0.3 M sucrose; treatment for 20 min at room temperature with LS containing 2.0 M glycerol and 0.4 M sucrose; and dehydration with PVS2 for 60 min at 0A degrees C. Under these conditions, 71.0% recovery of cryopreserved shoot tips was achieved. Only 29.2% regeneration was noted with the D cryo-plate protocol.
Plan de classement
Sciences du monde végétal [076] ; Biotechnologies [084]
Description Géographique
ARGENTINE
Localisation
Fonds IRD [F B010064146]
Identifiant IRD
fdi:010064146
Contact