@article{fdi:010064131,
  title = {{S}econdary metabolite localization by autofluorescence in living plant cells},
  author = {{T}alamond, {P}ascale and {V}erdeil, {J}. {L}. and {C}onejero, {G}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}utofluorescent molecules are abundant in plant cells and spectral images offer means for analyzing their spectra, yielding information on their accumulation and function. {B}ased on their fluorescence characteristics, an imaging approach using multiphoton microscopy was designed to assess localization of the endogenous fluorophores in living plant cells. {T}his method, which requires no previous treatment, provides an effective experimental tool for discriminating between multiple naturally-occurring fluorophores in living-tissues. {C}ombined with advanced {L}inear {U}nmixing, the spectral analysis extends the possibilities and enables the simultaneous detection of fluorescent molecules reliably separating overlapping emission spectra. {H}owever, as with any technology, the possibility for artifactual results does exist. {T}his methodological article presents an overview of the applications of tissular and intra-cellular localization of these intrinsic fluorophores in leaves and fruits (here for coffee and vanilla). {T}his method will provide new opportunities for studying cellular environments and the behavior of endogenous fluorophores in the intracellular environment.},
  keywords = {},
  booktitle = {},
  journal = {{M}olecules},
  volume = {20},
  numero = {3},
  pages = {5024--5037},
  ISSN = {1420-3049},
  year = {2015},
  DOI = {10.3390/molecules20035024},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064131},
}