@article{fdi:010064080,
  title = {{B}acterial diversity associated with wild caught anopheles mosquitoes from {D}ak {N}ong {P}rovince, {V}ietnam using culture and {DNA} fingerprint},
  author = {{N}go, {C}. {T}. and {A}ujoulat, {F}. and {V}eas, {F}rancisco and {J}umas-{B}ilak, {E}. and {M}anguin, {S}ylvie},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {M}icrobiota of {A}nopheles midgut can modulate vector immunity and block {P}lasmodium development. {I}nvestigation on the bacterial biodiversity in {A}nopheles, and specifically on the identification of bacteria that might be used in malaria transmission blocking approaches, has been mainly conducted on malaria vectors of {A}frica. {V}ietnam is an endemic country for both malaria and {B}ancroftian filariasis whose parasitic agents can be transmitted by the same {A}nopheles species. {N}o information on the microbiota of {A}nopheles mosquitoes in {V}ietnam was available previous to this study. {M}ethod {T}he culture dependent approach, using different mediums, and culture independent (16{S} r{RNA} {PCR} - {TTGE}) method were used to investigate the bacterial biodiversity in the abdomen of 5 {A}nopheles species collected from {D}ak {N}ong {P}rovince, central-south {V}ietnam. {M}olecular methods, sequencing and phylogenetic analysis were used to characterize the microbiota. {R}esults and {D}iscussion {T}he microbiota in wild-caught {A}nopheles was diverse with the presence of 47 bacterial {OTU}s belonging to 30 genera, including bacterial genera impacting {P}lasmodium development. {T}he bacteria were affiliated with 4 phyla, {A}ctinobacteria, {B}acteroidetes, {F}irmicutes and {P}roteobacteria, the latter being the dominant phylum. {F}our bacterial genera are newly described in {A}nopheles mosquitoes including {C}oxiella, {Y}ersinia, {X}anthomonas, and {K}noellia. {T}he bacterial diversity per specimen was low ranging from 1 to 4. {T}he results show the importance of pairing culture and fingerprint methods to better screen the bacterial community in {A}nopheles mosquitoes. {C}onclusion {S}ampled {A}nopheles species from central-south {V}ietnam contained a diverse bacterial microbiota that needs to be investigated further in order to develop new malaria control approaches. {T}he combination of both culture and {DNA} fingerprint methods allowed a thorough and complementary screening of the bacterial community in {A}nopheles mosquitoes.},
  keywords = {{VIET} {NAM}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {10},
  numero = {3},
  pages = {art. e0118634},
  ISSN = {1932-6203},
  year = {2015},
  DOI = {10.1371/journal.pone.0118634},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064080},
}