@article{fdi:010064042,
  title = {{G}enotoxicity of chemical compounds identification and assessment by yeast cells transformed with gfp reporter constructs regulated by the {PLM}2 or {DIN}7 promoter},
  author = {{B}ui, {V}. {N}. and {N}guyen, {T}. {T}. {H}. and {B}ettarel, {Y}van and {N}guyen, {T}. {H}. {T}. and {P}ham, {T}. {L}. and {H}oang, {T}. {Y}. and {N}guyen, {V}. {T}. {T}. and {N}ghiem, {N}. {M}. and {W}olfl, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{Y}east cells transformed with high-copy number plasmids comprising a green fluorescent protein ({GFP})-encoding gene optimized for yeast under the control of the new {DIN}7 or {PLM}2 and the established {RNR}2 and {RAD}54 promoters were used to assess the genotoxic potential of chemical compounds. {T}he activity of potential {DNA}-damaging agents was investigated by genotoxicity assays and by {O}xo{P}late assay in the presence of various test compounds. {T}he fluorescence signal generated by {GFP} in response to {DNA} damage was related to the different concentrations of analytes and the analyte-dependent {GFP} synthesis. {T}he use of distinct {DNA} damage-inducible promoters presents alternative genotoxicity testing strategies by selective induction of promoters in response to {DNA} damage. {T}he new {DIN}7 and {PLM}2 systems show higher sensitivity than the {RNR}2 and {RAD}54 systems in detecting 4-nitroquinoline-{N}-oxide and actinomycin {D}. {B}oth {DIN}7 and {PLM}2 systems are able to detect camptothecin while {RNR}2 and {RAD}54 systems are not. {A}utomated laboratory systems with assay performance on 384-well microplates provide for cost-effective high-throughput screening of {DNA}-damaging agents, reducing compound consumption to about 53% as compared with existing eukaryotic genotoxicity bioassays.},
  keywords = {biosensor ; {DNA}-damaging agents ; {DNA} damage-inducible promoters ; genotoxicity ; {GFP} ; {VIETNAM}},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {T}oxicology},
  volume = {34},
  numero = {1},
  pages = {31--43},
  ISSN = {1091-5818},
  year = {2015},
  DOI = {10.1177/1091581814566870},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064042},
}