Pyana P. P., Sere M., Kabore J., De Meeûs Thierry, MacLeod A., Bucheton Bruno, Van Reet N., Buscher P., Belem A. M. G., Jamonneau Vincent. (2015). Population genetics of Trypanosoma brucei gambiense in sleeping sickness patients with treatment failures in the focus of Mbuji-Mayi, Democratic Republic of the Congo. Infection Genetics and Evolution, 30, p. 128-133. ISSN 1567-1348.
Titre du document
Population genetics of Trypanosoma brucei gambiense in sleeping sickness patients with treatment failures in the focus of Mbuji-Mayi, Democratic Republic of the Congo
Année de publication
2015
Auteurs
Pyana P. P., Sere M., Kabore J., De Meeûs Thierry, MacLeod A., Bucheton Bruno, Van Reet N., Buscher P., Belem A. M. G., Jamonneau Vincent
Source
Infection Genetics and Evolution, 2015,
30, p. 128-133 ISSN 1567-1348
Human African trypanosomiasis (HAT) in the Democratic Republic of the Congo (DRC) is caused by the protozoan Trypanosoma brucei gambiense. Until recently, all patients in the second or neurological stage of the disease were treated with melarsoprol. At the end of the past and the beginning of the present century, alarmingly high relapse rates in patients treated with melarsoprol were reported in isolated HAT foci. In the Mbuji-Mayi focus of DRC, a particular mutation that confers cross resistance for pentamidine and melarsoprol was recently found for all strains studied. Nevertheless, treatment successfully cured a significant proportion of patients. To check for the existence of other possible genetic factors of the parasites, we genotyped trypanosomes isolated from patients before and after treatment (relapsing patients) with eight microsatellite markers. We found no evidence of any genetic correlation between parasite genotype and treatment outcome and we concluded that relapse or cure probably depend more on patients' factors such as disease progression, nutritional or immunological status or co-infections with other pathogens. The existence of a melarsoprol and pentamidine resistance associated mutation at such high rates highlights an increasing problem, even for other drugs, especially those using the same transporters as melarsoprol and pentamidine.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Santé : généralités [050]
;
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
REPUBLIQUE DEMOCRATIQUE DU CONGO
Localisation
Fonds IRD [F B010064041]
Identifiant IRD
fdi:010064041
