Publications des scientifiques de l'IRD

Jamonneau Vincent, Camara O., Ilboudo H., Peylhard M., Koffi M., Sakande H., N'Dri L., Sanou D., Dama E., Camara M., Lejon Veerle. (2015). Accuracy ofindividual rapid tests for serodiagnosis of gambiense sleeping sickness in west Africa. Plos Neglected Tropical Diseases, 9 (2), p. e0003480 [9 p.]. ISSN 1935-2735.

Titre du document
Accuracy ofindividual rapid tests for serodiagnosis of gambiense sleeping sickness in west Africa
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000350992500029
Auteurs
Jamonneau Vincent, Camara O., Ilboudo H., Peylhard M., Koffi M., Sakande H., N'Dri L., Sanou D., Dama E., Camara M., Lejon Veerle
Source
Plos Neglected Tropical Diseases, 2015, 9 (2), p. e0003480 [9 p.] ISSN 1935-2735
Background Individual rapid tests for serodiagnosis (RDT) of human African trypanosomiasis (HAT) are particularly suited for passive screening and surveillance. However, so far, no large scale evaluation of RDTs has been performed for diagnosis of Trypanosoma brucei gambiense HAT in West Africa. The objective of this study was to assess the diagnostic accuracy of 2 commercial HAT-RDTs on stored plasma samples from West Africa. Methodology/Principal findings SD Bioline HAT and HAT Sero-K-Set were performed on 722 plasma samples originating from Guinea and Cote d'Ivoire, including 231 parasitologically confirmed HAT patients, 257 healthy controls, and 234 unconfirmed individuals whose blood tested antibody positive in the card agglutination test but negative by parasitological tests. Immune trypanolysis was performed as a reference test for trypanosome specific antibody presence. Sensitivities in HAT patients were respectively 99.6% for SD Bioline HAT, and 99.1% for HAT Sero-K-Set, specificities in healthy controls were respectively 87.9% and 88.3%. Considering combined positivity in both RDTs, increased the specificity significantly (p <= 0.0003) to 93.4%, while 98.7% sensitivity was maintained. Specificities in controls were 98.7-99.6% for the combination of one or two RDTs with trypanolysis, maintaining a sensitivity of at least 98.1%. Conclusions/Significance The observed specificity of the single RDTs was relatively low. Serial application of SD Bioline HAT and HAT Sero-K-Set might offer superior specificity compared to a single RDT, maintaining high sensitivity. The combination of one or two RDTs with trypanolysis seems promising for HAT surveillance.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
GUINEE ; COTE D'IVOIRE
Localisation
Fonds IRD [F B010064004]
Identifiant IRD
fdi:010064004
Contact