@article{fdi:010063985, title = {{A} secondary dengue 4 infection in a traveler returning from {H}aiti confirmed by virus isolation, complete genome sequencing and neutralisation assay : a brief report}, author = {{M}enard, {A}. and {N}inove, {L}. and {Z}andotti, {C}. and {L}eparc-{G}offart, {I}. and {K}litting, {R}. and {B}aronti, {C}{\'e}cile and {S}tein, {A}. and de {L}amballerie, {X}avier and {C}harrel, {R}. {N}.}, editor = {}, language = {{ENG}}, abstract = {{H}ere we report the clinical and laboratory findings of a dengue 4 virus ({DENV}) secondary infection in a patient returning from {H}aiti to {F}rance. {T}he diagnostic of acute {DEN}-4 virus infection was demonstrated by (i) the presence of {DEN}-4 {RNA} in two successive serum samples, (ii) the isolation of a {DEN}-4 virus in {V}ero cells and subsequent identification of subtype {I}lb through complete genome sequencing, (iii) the presence of dengue {NS}1 antigen, (iv) the seroconversion with detection of dengue {I}g{M} in the second serum while negative in the first serum. {T}he diagnosis of secondary dengue episode was demonstrated by (i) the presence of dengue {I}g{G} in the early serum, and (ii) the demonstration that neutralising antibodies against {DEN}-3 were present at the acute stage of the disease. {N}ext-generation sequencing has a primary role to play in phylogeographic studies including database sequences, sequences from imported cases, and sequences from autochthonous cases.}, keywords = {{A}utochthonous ; {F}lavivirus ; {F}ever ; {T}raveler ; {I}mported ; {HAITI}}, booktitle = {}, journal = {{T}ravel {M}edicine and {I}nfectious {D}isease}, volume = {13}, numero = {1}, pages = {94--99}, ISSN = {1477-8939}, year = {2015}, DOI = {10.1016/j.tmaid.2014.12.009}, URL = {https://www.documentation.ird.fr/hor/fdi:010063985}, }