Menard A., Ninove L., Zandotti C., Leparc-Goffart I., Klitting R., Baronti Cécile, Stein A., de Lamballerie Xavier, Charrel R. N. (2015). A secondary dengue 4 infection in a traveler returning from Haiti confirmed by virus isolation, complete genome sequencing and neutralisation assay : a brief report. Travel Medicine and Infectious Disease, 13 (1), p. 94-99. ISSN 1477-8939.
Titre du document
A secondary dengue 4 infection in a traveler returning from Haiti confirmed by virus isolation, complete genome sequencing and neutralisation assay : a brief report
Menard A., Ninove L., Zandotti C., Leparc-Goffart I., Klitting R., Baronti Cécile, Stein A., de Lamballerie Xavier, Charrel R. N.
Source
Travel Medicine and Infectious Disease, 2015,
13 (1), p. 94-99 ISSN 1477-8939
Here we report the clinical and laboratory findings of a dengue 4 virus (DENV) secondary infection in a patient returning from Haiti to France. The diagnostic of acute DEN-4 virus infection was demonstrated by (i) the presence of DEN-4 RNA in two successive serum samples, (ii) the isolation of a DEN-4 virus in Vero cells and subsequent identification of subtype Ilb through complete genome sequencing, (iii) the presence of dengue NS1 antigen, (iv) the seroconversion with detection of dengue IgM in the second serum while negative in the first serum. The diagnosis of secondary dengue episode was demonstrated by (i) the presence of dengue IgG in the early serum, and (ii) the demonstration that neutralising antibodies against DEN-3 were present at the acute stage of the disease. Next-generation sequencing has a primary role to play in phylogeographic studies including database sequences, sequences from imported cases, and sequences from autochthonous cases.
