@article{fdi:010063957,
  title = {{G}enetic evolution of {M}ycobacterium bovis causing tuberculosis in livestock and wildlife in {F}rance since 1978},
  author = {{H}auer, {A}. and {D}e {C}ruz, {K}. and {C}ochard, {T}. and {G}odreuil, {S}. and {K}aroui, {C}. and {H}enault, {S}. and {B}ulach, {T}. and {B}anuls, {A}nne-{L}aure and {B}iet, {F}. and {B}oschiroli, {M}. {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}o study the dynamics of bovine tuberculosis (b{TB}) in {F}rance, 4,654 {M}. bovis strains isolated mainly from livestock and wildlife since 1978 were characterized by spoligotyping and {MLVA} based on {MIRU}-{VNTR}. {I}n our study spoligotyping allowed the discrimination of 176 types although 3 spoligotypes are predominant and account for more than half of the total strain population: {SB}0120 (26%), {SB}0134 (11%) and {SB}0121 (6%). {I}n addition, 11% of the isolates, principally from {S}outhern {F}rance, showing close spoligotypes and {MIRU}-{VNTR} types have been gathered in a family designated as the "{F}4-family". {MLVA} typing allowed extensive discrimination, particularly for strains with predominant spoligotypes, with a total of 498 genotypes, several of which were highly regionalized. {T}he similarity of the strains' genetic relationships based on spoligotyping and {MIRU}-{VNTR} markers supports the coexistence of different clonal populations within the {F}rench {M}. bovis population. {A} genetic evolution of the strains was observed both geographically and in time. {I}ndeed, as a result of the reduction of b{TB} due to the national control campaigns, a large reduction of the strains' genetic variability took place in the last ten years. {H}owever, in the regions were b{TB} is highly prevalent at present, cases in both livestock and in wildlife are due to the spread of unique local genotype profiles. {O}ur results show that the highly discriminating genotyping tools used in this study for molecular studies of b{TB} are useful for addressing pending questions, which would lead to a better insight into the epidemiology of the disease, and for finding proper solutions for its sustainable control in {F}rance.},
  keywords = {{FRANCE}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {10},
  numero = {2},
  pages = {e0117103 [17 p.]},
  ISSN = {1932-6203},
  year = {2015},
  DOI = {10.1371/journal.pone.0117103},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063957},
}