@article{fdi:010063948,
  title = {{C}omparison of matrix-assisted laser desorption ionization-time of flight mass spectrometry and molecular biology techniques for identification of {C}ulicoides ({D}iptera : {C}eratopogonidae) biting midges in {S}enegal},
  author = {{S}ambou, {M}. and {A}ubadie-{L}adrix, {M}. and {F}enollar, {F}. and {F}all, {B}. and {B}assene, {H}. and {A}lmeras, {L}. and {S}ambe-{B}a, {B}. and {P}errot, {N}. and {C}hatellier, {S}. and {F}aye, {N}. and {P}arola, {P}. and {W}ade, {B}. and {R}aoult, {D}idier and {M}ediannikov, {O}leg},
  editor = {},
  language = {{ENG}},
  abstract = {{B}iting midges of the genus {C}ulicoides are implicated as vectors for a wide variety of pathogens. {T}he morphological identification of these arthropods may be difficult because of a lack of detailed investigation of taxonomy for this species in {A}frica. {H}owever, matrix-assisted laser desorption ionization - time of flight mass spectrometry ({MALDI}-{TOF} {MS}) profiling is efficient for arthropod identification at the species level. {T}his study established a spectrum database of {C}ulicoides spp. from {S}enegal using {MALDI}-{TOF}. {I}dentification of {C}ulicoides insects to the species level before mass spectrometry was performed on the basis of morphological characters. {MALDI}-{TOF} {MS} reference spectra were determined for 437 field-caught {C}ulicoides of 10 species. {T}he protein profiles of all tested {C}ulicoides revealed several peaks with mass ranges of 2 to 20 k{D}a. {I}n a validation study, 72 {C}ulicoides specimens in the target species were correctly identified at the species level with a similarity of 95 to 99.9%. {F}our {C}ulicoides protein profiles were misidentified. {N}evertheless, six {S}uper{S}pectra ({C}. imicola, {C}. enderleini, {C}. oxystoma, {C}. kingi, {C}. magnus, and {C}. fulvithorax) were created. {A}bdomens of midges were used to amplify and sequence a portion of the mitochondrial cytochrome oxidase {I} gene ({COI}). {T}he results obtained using the {MALDI}-{TOF} {MS} method were consistent with the morphological identification and similar to the genetic identification. {P}rotein profiling using {MALDI}-{TOF} is an efficient approach for the identification of {C}ulicoides spp., and it is economically advantageous for approaches that require detailed and quantitative information of vector species that are collected in field. {T}he database of {A}frican {C}ulicoides {MS} spectra created is the first database in {A}frica. {T}he {COI} sequences of five {C}ulicoides species that were previously noncharacterized using molecular methods were deposited in {G}en{B}ank.},
  keywords = {{SENEGAL}},
  booktitle = {},
  journal = {{J}ournal of {C}linical {M}icrobiology},
  volume = {53},
  numero = {2},
  pages = {410--418},
  ISSN = {0095-1137},
  year = {2015},
  DOI = {10.1128/jcm.01855-14},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063948},
}