@article{fdi:010063914,
  title = {{A}nopheles gambiae salivary protein expression modulated by wild {P}lasmodium falciparum infection : highlighting of new antigenic peptides as candidates of {A}n. gambiae bites},
  author = {{M}arie, {A}. and {H}olzmuller, {P}. and {T}chioffo, {M}. {T}. and {R}ossignol, {M}arie and {D}emettre, {E}. and {S}eveno, {M}. and {C}orbel, {V}incent and {A}wono-{A}mbene, {P}. and {M}orlais, {I}sabelle and {R}emou{\'e}, {F}ranck and {C}orn{\'e}lie, {S}ylvie},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {M}alaria is the major parasitic disease worldwide caused by {P}lasmodium infection. {T}he objective of integrated malaria control programs is to decrease malaria transmission, which needs specific tools to be accurately assessed. {I}n areas where the transmission is low or has been substantially reduced, new complementary tools have to be developed to improve surveillance. {A} recent approach, based on the human antibody response to {A}nopheles salivary proteins, has been shown to be efficient in evaluating human exposure to {A}nopheles bites. {T}he aim of the present study was to identify new {A}n. gambiae salivary proteins as potential candidate biomarkers of human exposure to {P}. falciparum-infective bites. {M}ethods: {E}xperimental infections of {A}n. gambiae by wild {P}. falciparum were carried out in semi-field conditions. {T}hen a proteomic approach, combining 2{D}-{DIGE} and mass spectrometry, was used to identify the overexpressed salivary proteins in infected salivary glands compared to uninfected {A}n. gambiae controls. {S}ubsequently, a peptide design of each potential candidate was performed in silico and their antigenicity was tested by an epitope-mapping technique using blood from individuals exposed to {A}nopheles bites. {R}esults: {F}ive salivary proteins (g{SG}6, g{SG}1b, {TRIO}, {SG}5 and long form {D}7) were overexpressed in the infected salivary glands. {E}ighteen peptides were designed from these proteins and were found antigenic in children exposed to the {A}nopheles bites. {M}oreover, the results showed that the presence of wild {P}. falciparum in salivary glands modulates the expression of several salivary proteins and also appeared to induce post-translational modifications. {C}onclusions: {T}his study is, to our knowledge, the first that compares the sialome of {A}n. gambiae both infected and not infected by wild {P}. falciparum, making it possible to mimic the natural conditions of infection. {T}his is a first step toward a better understanding of the close interactions between the parasite and the salivary gland of mosquitoes. {I}n addition, these results open the way to define biomarkers of infective bites of {A}nopheles, which could, in the future, improve the estimation of malaria transmission and the evaluation of malaria vector control tools.},
  keywords = {{A}nopheles gambiae ; {W}ild {P}lasmodium falciparum ; {S}alivary proteins ; {B}iomarker ; {I}nfective bites ; {P}roteomic},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {7},
  numero = {},
  pages = {art. 599 [13 p.]},
  ISSN = {1756-3305},
  year = {2015},
  DOI = {10.1186/s13071-014-0599-y},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063914},
}