@article{fdi:010063903,
  title = {{E}xperimental study of the relationship between {P}lasmodium gametocyte density and infection success in mosquitoes : implications for the evaluation of malaria transmission-reducing interventions},
  author = {{D}a, {D}. {F}. and {C}hurcher, {T}. {S}. and {Y}erbanga, {R}. {S}. and {Y}ameogo, {B}. and {S}angare, {I}. and {O}uedraogo, {J}. {B}. and {S}inden, {R}. {E}. and {B}lagborough, {A}. {M}. and {C}ohuet, {A}nna},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he evaluation of transmission reducing interventions ({TRI}) to control malaria widely uses membrane feeding assays. {I}n such assays, the intensity of {P}lasmodium infection in the vector might affect the measured efficacy of the candidates to block transmission. {G}ametocyte density in the host blood is a determinant of the infection success in the mosquito, however, uncertain estimates of parasite densities and intrinsic characteristics of the infected blood can induce variability. {T}o reduce this variation, a feasible method is to dilute infectious blood samples. {W}e describe the effect of diluting samples of {P}lasmodium-containing blood samples to allow accurate relative measures of gametocyte densities and their impact on mosquito infectivity and {TRI} efficacy. {N}atural {P}lasmodium falciparum samples were diluted to generate a wide range of parasite densities, and fed to {A}nopheles coluzzii mosquitoes. {T}his was compared with parallel dilutions conducted on {P}lasmodium berghei infections. {W}e examined how blood dilution influences the observed blocking activity of anti-{P}bs28 monoclonal antibody using the {P}. berghei/{A}nopheles stephensi system. {I}n the natural species combination {P}. falciparum/{A}n. coluzzii, blood dilution using heat-inactivated, infected blood as diluents, revealed positive near linear relationships, between gametocyte densities and oocyst loads in the range tested. {A} similar relationship was observed in the {P}. berghei/{A}n. stephensi system when using a similar dilution method. {I}n contrast, diluting infected mice blood with fresh uninfected blood dramatically increases the infectiousness. {T}his suggests that highly infected mice blood contains inhibitory factors or reduced blood moieties, which impede infection and may in turn, lead to misinterpretation when comparing individual {TRI} evaluation assays. {I}n the lab system, the transmission blocking activity of an antibody specific for {P}bs28 was confirmed to be density-dependent. {T}his highlights the need to carefully interpret evaluations of {TRI} candidates, regarding gametocyte densities in the {P}. berghei/{A}n. stephensi system.},
  keywords = {{G}ametocyte density ; {I}nfection success ; {T}ransmission blocking ; interventions ; {D}ilution ; {P}lasmodium ; {A}nopheles ; {BURKINA} {FASO}},
  booktitle = {},
  journal = {{E}xperimental {P}arasitology},
  volume = {149},
  numero = {},
  pages = {74--83},
  ISSN = {0014-4894},
  year = {2015},
  DOI = {10.1016/j.exppara.2014.12.010},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063903},
}