@article{fdi:010063842,
  title = {{C}haracterization of {S}porohalobacter salinus sp nov., an anaerobic, halophilic, fermentative bacterium isolated from a hypersaline lake},
  author = {{B}en {A}bdallah, {M}. and {K}array, {F}. and {M}hiri, {N}. and {C}ayol, {J}. {L}. and {T}holozan, {J}. {L}. and {A}lazard, {D}idier and {S}ayadi, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{H}alophilic, obligately anaerobic, {G}ram-stain-negative bacterial strains were isolated from a sediment sample taken from under the salt crust of {E}l-{J}erid hypersaline lake in southern {T}unisia by using tryptone or glucose as the substrate. {O}ne strain, {CEJFT}1{B}({T}), was characterized phenotypically and phylogenetically. {C}ells were non-motile, non-spore-forming, short rods. {S}train {CEJFT}1{B}({T}) was able to grow in the presence of 5-30% (w/v) {N}a{C}l (optimum 20%) and at 30-60 degrees {C} (optimum 45 degrees {C}). {I}t grew at p{H} 5.5-7.8 and the optimum p{H} for growth was 6.8. {T}he isolate required yeast extract for growth. {S}ubstrates utilized by strain {CEJFT}1{B}({T}) as the sole carbon source included glucose, fructose, sucrose, pyruvate, {C}asamino acids and starch. {I}ndividual amino acids such as glutamate, lysine, methionine, serine, tyrosine, and amino acid mixtures formed by the {S}tickland reaction such as alanine-glycine, valine-proline, leucine-proline, isoleucine-proline were also utilized. {P}roducts of glucose fermentation were acetate (major product), butyrate, {H}-2 and {CO}2. {T}he genomic {DNA} {G}+{C} content of strain {CEJFT}1{B}({T}) was 32.3 mol%. {P}hylogenetic analysis based on 16{S} r{RNA} gene sequences indicated that strain {CEJFT}1{B}({T}) should be assigned to the genus {S}porohalobacter. {T}he sequence similarity between strain {CEJFT}1{B}({T}) and {S}porohalobacter lortetii was 98.5 %, but {DNA}-{DNA} hybridization between the two strains revealed a relatedness value of 56.4%, indicating that they are not related at the species level. {T}he combination of phylogenetic analysis, {DNA}-{DNA} hybridization data, and differences in substrate utilization support the view that strain {CEJFT}1{B}({T}) represents a novel species of the genus {S}porohalobacter, for which the name {S}porohalobacter salinus sp. nov. is proposed. {T}he type strain is {CEJFT}1{B}({T}) (={DSM} 26781({T})={JCM} 19279({T})).},
  keywords = {{TUNISIE}},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {S}ystematic and {E}volutionary {M}icrobiology},
  volume = {65},
  numero = {2},
  pages = {543--548},
  ISSN = {1466-5026},
  year = {2015},
  DOI = {10.1099/ijs.0.066845-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063842},
}