%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Larrieu, A.
%A Champion, Antony
%A Legrand, J.
%A Lavenus, J.
%A Mast, D.
%A Brunoud, G.
%A Oh, J.
%A Guyomarc'h, S.
%A Pizot, Maxime
%A Farmer, E. E.
%A Turnbull, C.
%A Vernoux, T.
%A Bennett, M. J.
%A Laplaze, Laurent
%T A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants
%D 2015
%L fdi:010063729
%G ENG
%J Nature Communications
%@ 2041-1723
%M ISI:000348830000005
%P art. 6043 [8 ]
%R 10.1038/ncomms7043
%U https://www.documentation.ird.fr/hor/fdi:010063729
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-10/010063729.pdf
%V 6
%W Horizon (IRD)
%X Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification of dynamic changes in JA distribution in response to stress with high spatiotemporal sensitivity. We show that Jas9-VENUS abundance is dependent on bioactive JA isoforms, the COI1 co-receptor, a functional Jas motif and proteasome activity. We demonstrate the utility of Jas9-VENUS to analyse responses to JA in planta at a cellular scale, both quantitatively and dynamically. This included using Jas9-VENUS to determine the cotyledon-to-root JA signal velocities on wounding, revealing two distinct phases of JA activity in the root. Our results demonstrate the value of developing quantitative sensors such as Jas9-VENUS to provide high-resolution spatiotemporal data about hormone distribution in response to plant abiotic and biotic stresses.
%$ 076 ; 020