@article{fdi:010063729,
  title = {{A} fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants},
  author = {{L}arrieu, {A}. and {C}hampion, {A}ntony and {L}egrand, {J}. and {L}avenus, {J}. and {M}ast, {D}. and {B}runoud, {G}. and {O}h, {J}. and {G}uyomarc'h, {S}. and {P}izot, {M}axime and {F}armer, {E}. {E}. and {T}urnbull, {C}. and {V}ernoux, {T}. and {B}ennett, {M}. {J}. and {L}aplaze, {L}aurent},
  editor = {},
  language = {{ENG}},
  abstract = {{A}ctivated forms of jasmonic acid ({JA}) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. {H}ere we describe a {JA} perception biosensor termed {J}as9-{VENUS} that allows the quantification of dynamic changes in {JA} distribution in response to stress with high spatiotemporal sensitivity. {W}e show that {J}as9-{VENUS} abundance is dependent on bioactive {JA} isoforms, the {COI}1 co-receptor, a functional {J}as motif and proteasome activity. {W}e demonstrate the utility of {J}as9-{VENUS} to analyse responses to {JA} in planta at a cellular scale, both quantitatively and dynamically. {T}his included using {J}as9-{VENUS} to determine the cotyledon-to-root {JA} signal velocities on wounding, revealing two distinct phases of {JA} activity in the root. {O}ur results demonstrate the value of developing quantitative sensors such as {J}as9-{VENUS} to provide high-resolution spatiotemporal data about hormone distribution in response to plant abiotic and biotic stresses.},
  keywords = {},
  booktitle = {},
  journal = {{N}ature {C}ommunications},
  volume = {6},
  numero = {},
  pages = {art. 6043 [8 p.]},
  ISSN = {2041-1723},
  year = {2015},
  DOI = {10.1038/ncomms7043},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063729},
}