@article{fdi:010063649,
  title = {{L}eishmanicidal compounds and potent {PPAR} gamma activators from {R}enealmia thyrsoidea ({R}uiz & {P}av.) {P}oepp. & {E}ndl},
  author = {{C}abanillas, {B}. {J}. and {L}e {L}amer, {A}. {C}. and {O}lagnier, {D}. and {C}astillo, {D}. and {A}revalo, {J}. and {V}aladeau, {C}. and {C}oste, {A}. and {P}ipy, {B}. and {B}ourdy, {G}enevi{\`e}ve and {S}auvain, {M}ichel and {F}abre, {N}icolas},
  editor = {},
  language = {{ENG}},
  abstract = {{E}thnopharmacological relevance: {L}eaves and rhizomes of {R}enealmia thyrsoidea ({R}uiz & {P}av.) {P}oepp. & {E}ndl. traditionally used in the {Y}anesha pharmacopoeia to treat skin infections such as leishmaniasis ulcers, or to reduce fever were chemically investigated to identify leishmanicidal compounds, as well as {PPAR} gamma activators. {M}ethods: {C}ompounds were isolated through a bioassay-guided fractionation and their structures were determined via detailed spectral analysis. {T}he viability of {L}eishmania amazonensis axenic amastigotes was assessed by the reduction of tetrazolium salt ({MIT}), the cytotoxicity on macrophage was evaluated using trypan blue dye exclusion method, while the percentage of infected macrophages was determined microscopically in the intracellular macrophage-infected assay. {T}he {CD}36, mannose receptor ({MR}) and dectin-1 m{RNA} expression on human monocytes-derived macrophages was evaluated by quantitative real-time {PCR}. {R}esults: {S}ix sesquiterpenes (1-6), one dihydrobenzofuranone (7) and four flavonoids (8-11) were isolated from the leaves. {A}longside, two flavonoids (12-13) and five diarylheptanoids (14-18) were identified in the. rhizomes. {L}eishmanicidal activity against {L}eishmania amazonensis axenic amastigotes was evaluated for all compounds. {C}ompounds 6, 7, and 11, isolated from the leaves, showed to be the most active derivatives. {D}iarylheptanoids 14-18 were also screened for their ability to activate {PPAR} gamma nuclear receptor in macrophages. {C}ompounds 17 and 18 bearing a {M}ichael acceptor moiety strongly increased the expression of {PPAR} gamma target genes such as {CD}36, {D}ectin-1 and mannose receptor ({MR}), thus revealing interesting immunomodulatory properties. {C}onclusions: {P}hytochemical investigation of {R}enealmia thyrsoidea has led to the isolation of leishmanicidal compounds from the leaves and potent {PPAR} gamma activators from the rhizomes. {T}hese results are in agreement with the traditional uses of the different parts of {R}enealmia thyrsoidea.},
  keywords = {{R}enealmia thyrsoidea ; {Z}ingiberaceae ; {L}eishmanicidal activity ; {PPAR} ; gamma ; {M}edicinal plants ; {P}eru ; {PEROU}},
  booktitle = {},
  journal = {{J}ournal of {E}thnopharmacology},
  volume = {157},
  numero = {},
  pages = {149--155},
  ISSN = {0378-8741},
  year = {2014},
  DOI = {10.1016/j.jep.2014.09.010},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063649},
}