@article{fdi:010063621,
  title = {{P}n{T}gs1-like expression during reproductive development supports a role for {RNA} methyltransferases in the aposporous pathway},
  author = {{S}iena, {L}. {A}. and {O}rtiz, {J}. {P}. {A}. and {L}eblanc, {O}livier and {P}essino, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {I}n flowering plants, apomixis (asexual reproduction via seeds) is widely believed to result from failure of key regulators of the sexual female reproductive pathway. {I}n the past few years, both differential display and {RNA}-seq comparative approaches involving reproductive organs of sexual plants and their apomictic counterparts have yielded extensive lists of candidate genes. {N}evertheless, only a limited number of these genes have been functionally characterized, with few clues consequently available for understanding the molecular control of apomixis. {W}e have previously identified several c{DNA} fragments with high similarity to genes involved in {RNA} biology and with differential amplification between sexual and apomictic {P}aspalum notatum plants. {H}ere, we report the characterization of one of these candidates, namely, {N}69 encoding a protein of the {S}-adenosyl-{L}-methionine-dependent methyltransferases superfamily. {T}he purpose of this work was to extend the {N}69 c{DNA} sequence and to characterize its expression at different developmental stages in both sexual and apomictic individuals. {R}esults: {M}olecular characterization of the {N}69 c{DNA} revealed homology with genes encoding proteins similar to yeast and mammalian trimethylguanosine synthase/{PRIP}-interacting proteins. {T}hese proteins play a dual role as {ERK}2-controlled transcriptional coactivators and mediators of sn(o){RNA} and telomerase {RNA} cap trimethylation, and participate in mammals and yeast development. {T}he {N}69-extended sequence was consequently renamed {P}n{T}gs1-like. {E}xpression of {P}n{T}gs1-like during reproductive development was significantly higher in floral organs of sexual genotypes compared with apomicts. {T}his difference was not detected in vegetative tissues. {I}n addition, expression levels in reproductive tissues of several genotypes were negatively correlated with facultative apomixis rates. {M}oreover, in situ hybridization observations revealed that {P}n{T}gs1-like expression is relatively higher in ovules of sexual plants throughout development, from premeiosis to maturity. {T}issues where differential expression is detected include nucellar cells, the site of aposporous initials differentiation in apomictic genotypes. {C}onclusions: {O}ur results indicate that {P}n{T}gs1-like (formerly {N}69) encodes a trimethylguanosine synthase-like protein whose function in mammals and yeast is critical for development, including reproduction. {O}ur findings also suggest a pivotal role for this candidate gene in nucellar cell fate, as its diminished expression is correlated with initiation of the apomictic pathway in plants.},
  keywords = {{A}pomixis ; {A}pospory ; {G}ene expression ; {PIMT} ; {RNA} processing ; {T}rimethylguanosine synthase},
  booktitle = {},
  journal = {{BMC} {P}lant {B}iology},
  volume = {14},
  numero = {},
  pages = {297 [10 ]},
  ISSN = {1471-2229},
  year = {2014},
  DOI = {10.1186/s12870-014-0297-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063621},
}