@article{fdi:010063619,
  title = {{I}dentification of potential transcriptional regulators of actinorhizal symbioses in {C}asuarina glauca and {A}lnus glutinosa},
  author = {{D}i{\'e}dhiou, {I}. and {T}romas, {A}lexandre and {C}issoko, {M}. and {G}ray, {K}rystelle and {P}arizot, {B}. and {C}rabos, {A}mandine and {A}lloisio, {N}. and {F}ournier, {P}. and {C}arro, {L}. and {S}vistoonoff, {S}ergio and {G}herbi, {H}assen and {H}ocher, {V}al{\'e}rie and {D}iouf, {D}. and {L}aplaze, {L}aurent and {C}hampion, {A}ntony},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}rees belonging to the {C}asuarinaceae and {B}etulaceae families play an important ecological role and are useful tools in forestry for degraded land rehabilitation and reforestation. {T}hese functions are linked to their capacity to establish symbiotic relationships with a nitrogen-fixing soil bacterium of the genus {F}rankia. {H}owever, the molecular mechanisms controlling the establishment of these symbioses are poorly understood. {T}he aim of this work was to identify potential transcription factors involved in the establishment and functioning of actinorhizal symbioses. {R}esults: {W}e identified 202 putative transcription factors by in silico analysis in 40 families in {C}asuarina glauca ({C}asuarinaceae) and 195 in 35 families in {A}lnus glutinosa ({B}etulaceae) {EST} databases. {B}ased on published transcriptome datasets and quantitative {PCR} analysis, we found that 39% and 26% of these transcription factors were regulated during {C}. glauca and {A}. glutinosa-{F}rankia interactions, respectively. {P}hylogenetic studies confirmed the presence of common key transcription factors such as {NSP}, {NF}-{YA} and {ERN}-related proteins involved in nodule formation in legumes, which confirm the existence of a common symbiosis signaling pathway in nitrogen-fixing root nodule symbioses. {W}e also identified an actinorhizal-specific transcription factor belonging to the zinc finger {C}1-2i subfamily we named {C}g{ZF}1 in {C}. glauca and {A}g{ZF}1 in {A}. glutinosa. {C}onclusions: {W}e identified putative nodulation-associated transcription factors with particular emphasis on members of the {GRAS}, {NF}-{YA}, {ERF} and {C}2{H}2 families. {I}nterestingly, comparison of the non-legume and legume {TF} with signaling elements from actinorhizal species revealed a new subgroup of nodule-specific {C}2{H}2 {TF} that could be specifically involved in actinorhizal symbioses. {I}n silico identification, transcript analysis, and phylogeny reconstruction of transcription factor families paves the way for the study of specific molecular regulation of symbiosis in response to {F}rankia infection.},
  keywords = {{T}ranscription factors ; {S}ymbiosis ; {A}ctinorhizal plants ; {S}ignaling pathway ; {C}2{H}2 transcription factors},
  booktitle = {},
  journal = {{BMC} {P}lant {B}iology},
  volume = {14},
  numero = {},
  pages = {342},
  ISSN = {1471-2229},
  year = {2014},
  DOI = {10.1186/s12870-014-0342-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063619},
}