@article{fdi:010063392,
  title = {{A}ssessment of various strategies for the preservation of clonal genetic resources in oil palm ({E}laeis guineensis {J}acq.)},
  author = {{K}onan, {K}.{E}. and {R}ival, {A}. and {K}ouadio, {Y}.{J}. and {D}uval, {Y}ves and {F}lori, {A}. and {A}don, {B}. and {P}ene, {C}. and {G}asselin, {T}.{D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}hree different approaches for the preservation of oil palm ({E}laeis guineensis {J}acq.) clonal genetic resources and their impacts on the induction of the « mantled » somaclonal variation were assessed. {I}n vitro long term preservation of somatic embryos stock-cultures was studied : after a 5 year cultivation period, 75 % of clonal lines were still normal. {B}etween 8 and 13 years of embryo cultures, half of the considered clonal lines were found to be « mantled ». {F}inally, 40 % were found to be normal over 15 years of in vitro conservation. {C}lonal conformity of ramets resulting from the re-cloning of somaplants depended, on one hand, on the floral status of the mother plant at the time of sampling and, on the other hand, on its origin. {R}e-cloning of abnormal regenerants led, in all cases, to 100 % abnormal offspring. {T}he age of the ramet used as mother palm at the time of sampling was found to be critical for true-to-type regeneration. {T}here is a high risk of obtaining variant regenerant palms if the clonal mother palm is sampled at nursery stage. {F}ield observations carried out on palms originating from somatic embryos cryopreserved at -196 °{C} showed floral conformity rates comparable to those obtained from standard not-cryopreserved clonal palms, for 6 out of the 8 clonal lines studied. {F}rom the 2 remaining clonal lines, a few regenerant palms originating from standard batch were found to be « mantled », whereas those resulting from cryopreserved embryos were all normal. {T}he assumption of changes in levels of genomic {DNA} methylation during preservation was discussed, together with the capacity of our cryopreservation protocol to select embryogenic cells which were only suited to trueto-type regeneration.},
  keywords = {{PALMIER} {A} {HUILE} ; {CONSERVATION} {DES} {RESSOURCES} {GENETIQUES} ; {EMBRYOGENESE} {SOMATIQUE} ; {CULTURE} {IN} {VITRO} ; {CLONE} ; {CRYOCONSERVATION} ; {COTE} {D}'{IVOIRE}},
  booktitle = {},
  journal = {{A}gronomie {A}fricaine},
  volume = {18},
  numero = {3},
  pages = {187--200},
  ISSN = {1015-2288},
  year = {2006},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063392},
}