@article{fdi:010063324,
  title = {{U}ltrastructural changes during cryopreservation of plumules and embryos of coconut ({C}ocos nucifera {L}.)},
  author = {{A}lla-{N}'{N}an, {O}. and {T}i{\'e}coura, {K}. and {G}on{\'e}del{\'e} {B}i, {S}. and {V}erdeil, {J}.{L}. and {M}alaurie, {B}ernard},
  editor = {},
  language = {{ENG}},
  abstract = {{T}his article aims to show the changes occurring during cryopreservation of embryos and plumules of coconut which are responsible of their death or survival. {E}mbryos have been cryopreserved by preculture-dehydration for 24h, 28h, 30h, 34h, 38h and 48h on agar medium containing 600g/{L} of glucose combined with silica gel. {T}he plumules were cryopreserved by encapsulation dehydration on solid medium containing 0.5 {M}, 0.75 {M} and 1{M} followed by dehydration with 40g of silica gel for different durations before rapid freezing. {T}his study indicates that the damages undergone by seed samples can be divided into three types. {T}he first stage of changes concerned the plasmolysis of cells with small vacuoles, condensation of chromatin, changing in the conformation of the {DNA} and the nucleus and stopping of mitosis. {T}hese types of changes are described in general in the context of a desiccation tolerance. {T}he second degree of the changes was the retraction of the cytoplasm inside the cell, the increase in the periplasmic volume. {T}he third degree of modification concerned the deformation of the walls, the invagination or the lysis of the plasma membrane resulting in the observation of distorted cells and and the bursting of the nucleus. {T}hese two types of modifications are irreversible and correspond to an absence of regrowth of the samples. {U}nderstanding the damage or changes that occur in cryopreserved cells is an important part of understanding how dehydration and frozen affect the viability of recalcitrant plants cells. {T}hese changes are made by dehydration and accentuated by freezing.},
  keywords = {{NOIX} {DE} {COCO} ; {CONSERVATION} {DES} {RESSOURCES} {GENETIQUES} ; {CULTURE} {IN} {VITRO} ; {DESYBRIDATION} ; {CONGELATION} ; {EMBRYON} {ZYGOTIQUE} ; {ULTRASTRUCTURE} ; {TAUX} {DE} {SURVIE} ; {CRYOCONSERVATION} ; {ZONE} {TROPICALE}},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {A}gronomy and {A}gricultural {R}esearch},
  volume = {5},
  numero = {6},
  pages = {103--115},
  ISSN = {2223-7054},
  year = {2014},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063324},
}