@article{fdi:010063159,
  title = {{R}ole of {CD}8(+) {T} cells in protection against {L}eishmania donovani infection in healed visceral leishmaniasis individuals},
  author = {{K}aushal, {H}. and {B}ras {G}oncalves, {R}achel and {N}egi, {N}. {S}. and {L}emesre, {J}ean-{L}oup and {P}apierok, {G}. and {S}alotra, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {M}ajority of individuals with history of visceral leishmaniasis ({VL}) exhibit strong immunity to re-infection, however, the mechanism of resistance is poorly understood. {I}t is unclear whether {CD}8(+) {T} cells contribute to protection against {L}eishmania donovani infection through cytotoxic activity. {T}he present study aims to evaluate immunological mechanism associated with resistance to the disease in healed {VL} ({HVL}) individuals and further, the contribution of {CD}8(+) {T} cells in the protective immunity. {M}ethods: {P}eripheral blood mononuclear cells ({PBMC}s) from {VL}, {HVL} and naive groups were exposed in vitro to total soluble {L}eishmania antigen ({TSLA}) from {L}. donovani. {T}he proliferation index was determined by {ELISA} based lymphoproliferative assay. {C}ytokines and granzyme {B} levels were measured by {CBA}. {A}ctivated {T}-cell populations were estimated using flow cytometry. {R}esults: {W}e observed significantly higher lymphoproliferation, cytokines and granzyme {B} levels in {HVL} group compared to naive or {VL} group. {M}ore strikingly, we found a strong association (r(s) = 0.895, {P} < 0.0001) between proliferation index ({PI}) and granzyme {B} level, with a significant proportion of activated {CD}8(+) {T} cells in {HVL} group. {C}onclusions: {L}eishmania immune group ({HVL}) exhibited durable and strong cellular immune response to {TSLA} in terms of lymphoproliferation as well as production of {T}h1 cytokines and granzyme {B}. {A}dditionally, the elevated level of activated {CD}8(+) {T} cells and stimulation of cytotoxic activity through granzyme {B} production, indicated a possible role of {CD}8(+) {T} cells in resistance to {L}. donovani infection in the {HVL} group.},
  keywords = {{CD}8(+) {T} cells ; {G}ranzyme {B} ; {V}isceral leishmaniasis ; {T}otal soluble ; {L}eishmania antigens ; {V}accine ; {INDE}},
  booktitle = {},
  journal = {{B}mc {I}nfectious {D}iseases},
  volume = {14},
  numero = {},
  pages = {art. 653 [7 p.]},
  ISSN = {1471-2334},
  year = {2014},
  DOI = {10.1186/s12879-014-0653-6},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063159},
}