@article{fdi:010063139,
  title = {{C}oconut (cocos nucifera l.) pollen cryopreservation},
  author = {{K}arun, {A}. and {S}ajini, {K}. {K}. and {N}iral, {V}. and {A}marnath, {C}. {H}. and {R}emya, {P}. and {R}ajesh, {M}. {K}. and {S}amsudeen, {K}. and {J}erard, {B}. {A}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{BACKGROUND}: {C}oconut genetic resources are threatened by pests and pathogens, natural hazards and human activities. {C}ryopreservation is the only method allowing the safe and cost-effective long-term conservation of recalcitrant seed species such as coconut. {OBJECTIVE}: {T}he objective of this work was to test the effect of cryopreservation and of cryostorage duration on coconut pollen germination and fertility. {MATERIALS} {AND} {METHODS}: {P}ollen of two coconut varieties ({W}est {C}oast {T}all [{WCT}] and {C}howghat {O}range {D}warf [{COD}]) was collected in {M}arch-{M}ay over three successive years, desiccated to 7.5% moisture content ({FW}) and cryopreserved by direct immersion in liquid nitrogen. {RESULTS}: {G}ermination and pollen tube length ({PTL}) of desiccated and cryopreserved pollen were not significantly different for both {WCT} and {COD} over the three harvest months of the three consecutive years of study. {P}ollen germination ranged from 24 to 32% in desiccated pollen whereas it was between 26 and 29% in cryopreserved {COD} pollen. {I}n the case of {WCT}, germination ranged from 30 to 31% in desiccated pollen, while it was between 28 and 32% in cryopreserved pollen. {PTL} of cryopreserved pollen ranged between 224-390 mu m and 226-396 mu m for {COD} and {WCT}, respectively. {G}ermination of {COD} pollen varied between 29.0 and 44.1% after 4 years and 1.0/1.5 years cryostorage, respectively. {G}ermination of {WCT} pollen did not change significantly between 0 and 6 years cryostorage, being comprised between 32 (24 h) and 40 % (1.5 years). {G}ermination and vigour of cryopreserved pollen were generally higher compared to that of pollen dried in oven and non-cryopreserved. {N}ormal seed set was observed in {COD} and {WCT} palms using pollen cryostored for 6 months and 4 years. {C}ryopreserved pollen of five {T}all and five {D}warf accessions displayed 24-31% and 25-49% germination, respectively. {CONCLUSION}: {T}hese results show that it is now possible to establish pollen cryobanks to contribute to coconut germplasm long-term conservation.},
  keywords = {coconut ; pollen ; cryopreservation ; germination ; vigour ; fertility},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {35},
  numero = {5},
  pages = {407--417},
  ISSN = {0143-2044},
  year = {2014},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063139},
}