@article{fdi:010063095,
  title = {{C}omparative gene expression of {W}igglesworthia inhabiting non-infected and {T}rypanosoma brucei gambiense-infected {G}lossina palpalis gambiensis flies},
  author = {{S}oumana, {I}. {H}. and {T}chicaya, {B}. and {S}imo, {G}. and {G}eiger, {A}nne},
  editor = {},
  language = {{ENG}},
  abstract = {{T}setse flies ({G}lossina sp.) that transmit trypanosomes causing human (and animal) {A}frican trypanosomiasis ({HAT} and {AAT}, respectively) harbor symbiotic microorganisms, including the obligate primary symbiont {W}igglesworthia glossinidia. {A} relationship between {W}igglesworthia and tsetse fly infection by trypanosomes has been suggested, as removal of the symbiont results in a higher susceptibility to midgut infection in adult flies. {T}o investigate this relationship and to decipher the role of {W}. glossinidia in the fly's susceptibility to trypanosome infection, we challenged flies with trypanosomes and subsequently analyzed and compared the transcriptomes of {W}. glossinidia from susceptible and refractory tsetse flies at three time points (3, 10, and 20 days). {M}ore than 200 {W}. glossinidia genes were found to be differentially expressed between susceptible and refractory flies. {T}he high specificity of these differentially expressed genes makes it possible to distinguish {W}igglesworthia inhabiting these two distinct groups of flies. {F}urthermore, gene expression patterns were observed to evolve during the infection time course, such that very few differentially expressed genes were found in common in {W}igglesworthia from the 3-, 10- and 20-day post-feeding fly samples. {T}he overall results clearly demonstrate that the taking up of trypanosomes by flies, regardless of whether flies proceed with the developmental program of {T}rypanosoma brucei gambiense, strongly alters gene expression in {W}igglesworthia. {T}hese results therefore provide a novel framework for studies that aim to decrease or even abolish tsetse fly vector competence.},
  keywords = {{W}igglesworthia ; tsetse fly ; trypanosomes ; tripartite interactions ; transcriptome ; {AFRIQUE} {DE} {L}'{OUEST} ; {AFRIQUE} {DE} {L}'{EST} ; {COTE} {D}'{IVOIRE}},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {5},
  numero = {},
  pages = {art. 620 [16 p.]},
  ISSN = {1664-302{X}},
  year = {2014},
  DOI = {10.3389/fmicb.2014.00620},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063095},
}