@article{fdi:010063094,
  title = {{M}idgut expression of immune-related genes in {G}lossina palpalis gambiensis challenged with {T}rypanosoma brucei gambiense},
  author = {{S}oumana, {I}. {H}. and {T}chicaya, {B}. and {C}huchana, {P}. and {G}eiger, {A}nne},
  editor = {},
  language = {{ENG}},
  abstract = {{T}setse flies from the subspecies {G}lossina morsitans morsitans and {G}lossina palpalis gambiensis, respectively, transmit {T}rypanosoma brucei rhodesiense and {T}rypanosoma brucei gambiense. {T}he former causes the acute form of sleeping sickness, and the latter provokes the chronic form. {A}lthough several articles have reported {G}. m. morsitans gene expression following trypanosome infection, no comparable investigation has been performed for {G}. p. gambiensis. {T}his report presents results on the differential expression of immune-related genes in {G}. p. gambiensis challenged with {T}. b. gambiense. {T}he aim was to characterize transcriptomic events occurring in the tsetse gut during the parasite establishment step, which is the crucial first step in the parasite development cycle within its vector. {T}he selected genes were chosen from those previously shown to be highly expressed in {G}. m. morsitans, to allow further comparison of gene expression in both {G}lossina species. {U}sing quantitative {PCR}, genes were amplified from the dissected midguts of trypanosome-stimulated, infected, non-infected, and self-cleared flies at three sampling timepoints (3, 10, and 20 days) after a bloodmeal. {A}t the 3-day sampling point, transferrin transcripts were significantly up-regulated in trypanosome-challenged flies versus flies fed on non-infected mice. {I}n self-cleared flies, serpin-2 and thioredoxin peroxidase-3 transcripts were significantly up-regulated 10 days after trypanosome challenge, whereas nitric oxide synthase and chitin-binding protein transcripts were up-regulated after 20 days. {A}lthough the expression levels of the other genes were highly variable, the expression of immune-related genes in {G}. p. gambiensis appears to be a time-dependent process. {T}he possible biological significance of these findings is discussed, and the results are compared with previous reports for {G}. m. morsitans.},
  keywords = {{G}lossina palpalis gambiensis ; {T}rypanosoma brucei gambiense infection ; midgut ; immune gene expression ; {AFRIQUE} {DE} {L}'{OUEST} ; {COTE} {D}'{IVOIRE} ; {BURKINA} {FASO}},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {5},
  numero = {},
  pages = {art. 609 [7 p.]},
  ISSN = {1664-302{X}},
  year = {2014},
  DOI = {10.3389/fmicb.2014.00609},
  URL = {https://www.documentation.ird.fr/hor/fdi:010063094},
}