@article{fdi:010062998,
  title = {{I}solation of phytoplasma {DNA} from the coconut palms ({C}ocos nucifera {L}.) collected from {G}hana},
  author = {{A}lla-{N}'{N}an, {O}. and {G}ond{\'e}l{\'e} {B}i, {E}. and {K}ouassi, {A}.{B}. and {K}ouassi, {A}. and {P}hilippe, {R}. and {M}alaurie, {B}ernard and {D}ollet, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}his study aimed to verify the presence of the causative agent of {L}ethal {Y}ellowing which is phytoplasma in samples provided from infected coconut trees. {S}tudy was carried out by using various samples like zygotic embryo, young leaves and immature & mature inflorescences. {T}hese materials were collected from trees at the stage 1 and 2 of the disease development.. {S}tage 1 of disease development is characterized by leaf yellowing and the start of the falling nuts while at the stage 2 of disease development, the trees has not bear nuts longer. {F}rom infected material, {DNA} was extracted by three different processes and isolated {DNA} was amplified by {PCR}. 16{S} r{RNA} gene was amplified by two specific primers of phytoplama viz {P}1/{P}2 and {G}hana 813/{AKSR}. {A}mong the various tested materials presence of phytoplasma was reported from the mature inflorescences while the presence of the phytoplasma was not reported from the leaves and embryos of the coconut.},
  keywords = {{NOIX} {DE} {COCO} ; {CONSERVATION} {DES} {RESSOURCES} {GENETIQUES} ; {EMBRYON} {ZYGOTIQUE} ; {MALADIE} {DES} {PLANTES} ; {PATHOLOGIE} {VEGETALE} ; {AGENT} {PATHOGENE} ; {ADN} ; {COCOTIER} ; {GHANA}},
  booktitle = {},
  journal = {{J}ournal of {E}xperimental {B}iology and {A}gricultural {S}ciences},
  volume = {2},
  numero = {5},
  pages = {495--500},
  ISSN = {2320-8694},
  year = {2014},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062998},
}